Rapid identification and discrimination among Egyptian genotypes of Rhizobium leguminosarum bv. viciae and Sinorhizobium meliloti nodulating faba bean (Vicia faba L.) by analysis of nodC, ARDRA, and rDNA sequence analysis |
| |
Authors: | Abdelaal Shamseldin Muhammad El-Saadani Chung Sun An |
| |
Institution: | a Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technological Applications, New Borg El-Arab, Alexandria, Egypt b School of Biological Science, College of Natural Science, Seoul National University, Seoul, South Korea c Department of Soil, Water, and Climate, University of Minnesota, St. Paul, MN, USA d Biotechnology Institute, University of Minnesota, St. Paul, MN, USA |
| |
Abstract: | Twenty-eight Rhizobium strains were isolated from the root nodules of faba bean (Vicia faba L.) collected from 11 governorates in Egypt. A majority of these strains (57%) were identified as Rhizobium leguminosarum bv. viciae (Rlv) based on analysis of a nodC gene fragment amplified using specific primers for these faba bean symbionts. The strains were characterized using a polyphasic approach, including nodulation pattern, tolerance to environmental stresses, and genetic diversity based on amplified ribosomal DNA-restriction analysis (ARDRA) of both 16S and 23S rDNA. Analysis of tolerance to environmental stresses revealed that some of these strains can survive in the presence of 1% NaCl and a majority of them survived well at 37 °C. ARDRA indicated that the strains could be divided into six 16S rDNA genotypes and five 23S rDNA genotypes. Sequence analysis of 16S rDNA indicated that 57% were Rlv, two strains were Rhizobium etli, one strain was taxonomically related to Rhizobium rubi, and a group of strains were most closely related to Sinorhizobium meliloti. Results of these studies indicate that genetically diverse rhizobial strains are capable of forming N2-fixing symbiotic associations with faba bean and PCR done using nodC primers allows for the rapid identification of V. faba symbionts. |
| |
Keywords: | Egyptian soils Faba bean Genetic diversity nodC Rlv Salinity and alkalinity of soils Sinorhizobium meliloti |
本文献已被 ScienceDirect 等数据库收录! |
|