甜菜潜隐病毒的研究

 从市场出售的甜菜(Beta vulgaris)种子中首次得到了直径约30毫微米的等轴病毒粒子的分离物。接种试验证明,它不能由摩擦接种传病,也不能由桃蚜(Myzus persicae)传播,但可由种子传毒,种子带毒率高达87.5%。受侵染的甜菜植株不表现症状,体内含毒量很低,病株汁液须经部分提纯并浓缩后才能在电镜下观察到病毒粒子和用琼脂双扩散法检测到病毒。在病株叶片的超薄切片中,未发现病毒粒子和细胞学的变异。在氯化铯溶液中,病毒粒子的浮力密度为1.37克/毫升左右。该分离物能与甜菜潜隐病毒抗血清产生沉淀反应,但与黄瓜花叶病毒无血清学关系。根据上述基本性状,该分离物归属于甜菜潜隐病毒(Beet Cryptic Virus)。

ON THE BEET CRYPTIC VIRUS OF SUGAR BEET

One isometric virus was first found from commercial seeds of sugar beet (Beta vulgaris) in China.The virus was unable to be transmitted by mechanical inoculation of beet sap and aphid inoculatoin using Myzus persicae,but was seed-transmitted to a large proportion of seedlings.87.5% of plants raised from commercial beet seeds were found to be infected.Infected plants were symptomless.The virus was present at a very low concentration in beets and was detected by electron microscopy of concentrated preparations made from individual plants.
The virus preparation did not react with antisera of cucumber mosaic virus but was serologically related to beet cryptic virus,usually forming a single precipitation line in gel immuno-diffusion tests.Purified preparation showed average particle diameter of 30 nm.After isopycnic centrifugation in CsCl solution,the virus preparation formed a main band of mean density 1.376g/ml and two fainter bands.In ultra thin sections of beets no virus-like particles or no cytological abnormalities were observed.On basis of the above stated properties,it is primarilly concluded that the virus belongs to Beet Cryptic Virus reported by Kassanis(1977).