民猪NUMB基因克隆与组织表达分析

研究采用RT-PCR技术结合克隆测序方法,克隆出全长2 004 bp NUMB基因序列,其中包括1 782 bp开放读码框,该基因编码592个氨基酸。利用生物信息学分析软件对该基因氨基酸序列预测与分析发现:NUMB蛋白二级结构中,转角占很大比例,且蛋白亲水性较强,结构功能域分析发现该蛋白多肽链存在一个N端磷酸酪氨酸结构域(PTB),位于第37~162氨基酸之间。基于不同物种NUMB蛋白序列所构建分子进化树表明,猪与牛、骆驼亲缘关系最近,而与斑马鱼最远。在m RNA水平上,NUMB基因肺中表达量最高,大肠中表达量最低。

Cloning and tissue expression analysis of Min pig NUMB gene

In this study, RT-PCR technology combined with cloning and sequencing methods, cloned the full-length gene sequence NUMB 2 004 bp, including 1 782 bp open reading frame (ORF), which encodes 592 amino acids. Bioinformatics analysis software for prediction and analysis of the amino acid sequence of the gene discovery: NUMB protein secondary structure, a large proportion of the corner, and the proteins more hydrophilic, structural domains of the protein peptides found after analysis there was a chain of N-terminal tyrosine phosphatase domain (PTB), located between the first amino acid 37-162. Constructed based on different species NUMB protein sequence phylogenetic tree showed that kinship pigs with cattle and camels recently, with the zebra fish furthest. At the mRNA level, NUMB had highest gene expression in the lung, lowest expression in colon.