| 深黄被孢霉突变菌株发酵制备花生四烯酸工艺研究 |
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| 引用本文： | 杨勇,王中江,毕爽,张辉,李杨,江连洲.深黄被孢霉突变菌株发酵制备花生四烯酸工艺研究[J].东北农业大学学报,2016(1):45-50. |
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| 作者姓名： | 杨勇 王中江 毕爽 张辉 李杨 江连洲 |
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| 作者单位： | 1. 东北农业大学食品学院,哈尔滨 150030;齐齐哈尔大学食品与生物工程学院,农产品加工黑龙江省普通高校重点实验室,黑龙江 齐齐哈尔 161006;2. 东北农业大学食品学院,哈尔滨,150030;3. 科技部中国农业技术开发中心,北京,100045 |
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| 摘 要： | 在单因素试验基础上,采用响应面分析法优化深黄被孢霉突变菌株发酵制备花生四烯酸工艺,确定最优发酵工艺条件为:接种量15.66%,发酵温度28.29℃,发酵时间6.58 d,发酵p H 5.96。花生四烯酸产量为3.12 g·L-1,在最优发酵工艺条件下,可提高产量,同时减少接种量,缩短发酵时间,降低生产成本。
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| 关 键 词： | 深黄被孢霉 突变菌株 发酵 花生四烯酸 |
Research on preparation technology for arachidonic acid by fermentation of Mortierella isabellina mutant strain |
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| Abstract: | On the basis of single factor experiment, technological parameters of preparation for arachidonic acid by Mortierella isabellina mutant strain were determined by response surface methodology to optimize fermentation conditions as following: inoculum size was 15.66%, fermentation temperature was 28.29 ℃, fermentation time was 6.58 d, fermentation pH was 5.96. In the best fermentation process conditions, ARA yield was 3.12 g·L-1, which enhanced yield. At the same time, inoculum size was decreased, fermentation time was shortened, and production cost was reduced. |
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| Keywords: | Mortierella isabellina mutant strain fermentation arachidonic acid |
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