J亚群白血病病理学观察及抗原定位

通过病理组织学和免疫组织化学技 术对1日龄感染鸡定期进行病理组织学观察 及gp85病毒糖蛋白检测,确定病理变化和J 亚群白血病的组织学嗜性及二者的关系。结 果在3周龄时出现第1只发病鸡,以后所有 鸡发病且病变随着日龄的增加而加重,主要 在骨髓、肝脏、脾脏、肾脏、卵巢、心肌等组织 中出现瘤细胞增生,并有炎性反应;免疫组在 3周龄时,肝脏、心脏、肺脏、小肠、肾脏、卵巢、 骨骼肌开始出现阳性反应,抗原主要在胞浆 中出现,呈深蓝色颗粒。在10周龄～14周龄 时,骨髓呈中度的阳性反应,而其它组织的阳 性信号出现的程度未见大的变化;在23周龄 时,肝内肿瘤呈较强阳性反应,信号主要集中 在细胞核;整个实验过程中,法氏囊、脑始终 呈阴性反应。研究发现,骨髓及肿瘤的抗原 性较强,并且肿瘤的抗原性要强于骨髓;另外 还发现,在阳性反应强的组织中,病变较明 显,肿瘤细胞增生明显,即阳性反应的组织器 官易形成肿瘤转移灶。

Pathology Observation and Antigen Location of Avian leukosis virus subgroup J(ALV-J)

Two experimental groups were designed. Group 1: 1 d were inoculated with Inner Mongolia strain NM8761 of ALV-J. Another group was control. Histological samples were obtained from each chicken at weeks 3, 10, 14, 23,fixed in formalin. Three chickens were necropsied and tissuses were obtained for histopathology observation and for immunohistochemistry(IHC) examination. Results: typical lesion appeared at 3 weeks, and granulated myelocytes proliferated in bone marrow, liver, kidney, overy, heart muscle. These tissue companied severe inflammation.IHC: liver, heart, lung, intestines, kidney, overy, bone muscle had certain positive reaction, and antigens were detected in cytoplasm and nuclear, and the particles color were blue-purple, while fabricus and brain were negative reaction. Tumor cells in liver showed strong positive at weeks 23. This paper discovered that bone marrow and tumor had strong antigenicity.Another discovery was positive reaction of IHC had relationship with tissue which had typical lesion.