| 抗鸡传染性法氏囊病毒单克隆抗体的提纯和鉴定 |
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| 引用本文： | 陆苹,倪学英,胡志贤,王欣,黄薇.抗鸡传染性法氏囊病毒单克隆抗体的提纯和鉴定[J].上海交通大学学报(农业科学版),1994(1). |
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| 作者姓名： | 陆苹 倪学英 胡志贤 王欣 黄薇 |
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| 作者单位： | 上海农学院生物技术研究所 |
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| 基金项目： | 上海市科技兴农重点攻关项目,上海市农委资助 |
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| 摘 要： | 取抗鸡传染性法氏囊病毒单克隆抗体．经饱和硫酸铵盐析，透析除盐、葡聚糖凝胶过滤粗提后，经ＤＥＡＥ—２２纤维素离子交换层析，获得纯化的单抗，ＥＬＩＳＡ效价达１０（－６）以上．双向琼扩效价达１：６４以上；抗体回收率达６０％以上；最高浓度达１．２ｍｇ／ｍｌ．经抗鼠全血清双向琼扩结果证明所提取物为纯一的ＩｇＧ．
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| 关 键 词： | 单克隆抗体 葡聚糖凝胶层析 DEAE纤维素离子交换层析 酶联免疫测定 |
THE PURIFICATION AND IDENTIFICATION OF MONOCLONAL ANTIBODIES TO IBDV |
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| Lu Ping,Ni Xueying,Hu Zhixan,Wang Xing,Huang Wei.THE PURIFICATION AND IDENTIFICATION OF MONOCLONAL ANTIBODIES TO IBDV[J].Journal of Shanghai Jiaotong University (Agricultural Science),1994(1). |
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| Authors: | Lu Ping Ni Xueying Hu Zhixan Wang Xing Huang Wei |
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| Abstract: | Monoclonal Antibody to TBDV was precipitated by saturated ammonium sulphate solution, removed saline by dialysising and rough purified by Sephdcx-50 Gel Chromatography methods. After McAb was purified by DEAE-22 Cellulose Column Chromatography methods, we gained purific McAb whose titer by ELISA was above 10-6 and titer by two dimentioned immunodiffusion test in Gel was above 1:64. The rate of recovery was above 60%. The highest concentration of McAb to IBDV was 1 .2mg / ml. It was identified that the eintc was purific IgG as the result of two dimentioned immundiffusion test of anti-mice serum. |
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| Keywords: | Monoconal Antibody Sephdcx Gel Chromatography DEAE Cellulose Chromatography ELISA |
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