Canine adipose-derived-mesenchymal stem cells do not lose stem features after a long-term cryopreservation |
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Authors: | T. Martinello L. Maccatrozzo M. Sampaolesi M. Decaminada |
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Affiliation: | a Department of Experimental Veterinary Sciences, University of Padova, Italy b Department of Clinical Veterinary Sciences, University of Padova, Italy c Stem Cell Research Institute, University Hospital Gasthuisberg, Leuven, Belgium |
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Abstract: | Adult stem cells are nowadays used for treating several pathologies. A putative stem cell population was found in the adipose tissue of mammals and canine adipose tissue-derived-mesenchymal stem cells (cA-MSC) have been shown to possess the capacity to differentiate into several lineages. The main goal of our research was to fully characterize cA-MSC and examine the effects of cryopreservation on their stemness features. Each sample of cA-MSC was analyzed immediately and then again after being frozen in liquid nitrogen for one year. After the cryopreservation period cells conserved their fibroblast-like morphology, alkaline phosphatase positivity and CD expression but showed a lower proliferation ratio and a lower telomerase activity in comparison with fresh cells. Finally, the cryopreservation protocol did not change the cA-MSC adipogenic, osteogenic and myogenic differentiative potential. Our data demonstrate that stored cA-MSC might represent a promising type of progenitor cell for autologous cellular-based therapies in veterinary medicine. |
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Keywords: | Mesenchymal stem cells Cryopreservation Adipose tissue Stem cell differentiation Cell-surface markers |
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