| 猪圆环病毒2型ORF2基因在PcDNA3.1中的表达效果 |
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| 引用本文： | 车勇良,石运通,庄向生,陈少莺,王隆柏,魏宏,陈如敬,吴学敏,周伦江.猪圆环病毒2型ORF2基因在PcDNA3.1中的表达效果[J].福建农业学报,2011,26(2):166-169. |
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| 作者姓名： | 车勇良 石运通 庄向生 陈少莺 王隆柏 魏宏 陈如敬 吴学敏 周伦江 |
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| 作者单位： | 福建省农业科学院畜牧兽医研究所; |
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| 基金项目： | 福建省科技计划重点项目(2006N0026) |
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| 摘 要： | 目的]构建猪圆环病毒2型(Porcine circovirus 2,PCV2)ORF2 DNA核酸疫苗,并评价其免疫效果和安全性.方法]设计一对特异性引物,扩增PCV2-ORF2,利用T载体对其进行克隆,与真核表达载体PcDNA3.1在同等条件下进行双酶切,并进行连接,转化感受态细菌DH5a并克隆,经双酶切鉴定筛选...
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| 关 键 词： | 猪圆环病毒2型 ORF2 DNA疫苗 PcDNA3.1 |
Effect of Porcine circovirus Type 2 ORF2 Gene Expression in PcDNA3.1 for Vacination |
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| CHE Yong-liang,SHI Yun-tong,ZHUANG Xiang-sheng,CHEN Shao-ying,WANG Long-bai,WEI -Hong,CHEN Ru-jing,WU Xue-min,ZHOU Lun-jiang.Effect of Porcine circovirus Type 2 ORF2 Gene Expression in PcDNA3.1 for Vacination[J].Fujian Journal of Agricultural Sciences,2011,26(2):166-169. |
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| Authors: | CHE Yong-liang SHI Yun-tong ZHUANG Xiang-sheng CHEN Shao-ying WANG Long-bai WEI -Hong CHEN Ru-jing WU Xue-min ZHOU Lun-jiang |
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| Institution: | CHE Yong-liang,SHI Yun-tong,ZHUANG Xiang-sheng,CHEN Shao-ying,WANG Long-bai,WEI Hong,CHEN Ru-jing,WU Xue-min,ZHOU Lun-jiang(Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fujian Fuzhou 350013,China) |
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| Abstract: | This study was to develop ORF2 DNA vaccine against PCV2 and evaluate its immunity effect and safety.Method] A pair of specific primers was synthesized to amplify the PCV2-ORF2 by PCR.PCV2-ORF2 was cloned into T vector and named T-ORF2.T-ORF2 and PcDNA3.1 were double digested under same conditions,connected by using T4 DNA ligase,and cloned.The positive recombinant plasmids(DNA vaccine) were confirmed by double digestion and named PcDNA3.1-ORF2.The DNA vaccine was transfected in Vero cells with lipofectamin... |
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| Keywords: | Porcine circovirus type 2 ORF2 DNA vaccine PcDNA3 1 |
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