外源ABA对低温下雷公藤幼苗的生理响应

以1年生的雷公藤扦插苗为试验材料,对其外施浓度分别为5、10、15、20、25 mg·L-1脱落酸(ABA)溶液,以喷施蒸馏水为对照(CK),并进行连续7d的低温胁迫处理.研究低温胁迫下ABA对雷公藤幼苗叶片质膜透性、丙二醛(MDA)、保护酶和脯氨酸等生理生化指标的影响.研究表明,喷施浓度为15-20 mg·L-1的A...

Physiological response of exogenous ABA to Tripterygium wilfordii seedlings at low temperature

One year-old Tripterygium wilfordii cutting was used as the experiment material.By infliction abscisic acid(ABA) 5,10,15,20,25 mg · L-1 continuously 7 days under low temperature stress,and with the plant spraying distilled water as control treatment,the effects of ABA on membrane permeability,malondialdehyde(MDA) content,protective enzyme activity and proline content were studied in T.wilfordii seedlings leaves.The results showed that spraying 15-20 mg · L-1 ABA improved chilling resistance,effectively reducing relative electrical conductivity and the accumulation of MDA,which increased free proline content of T.wilfordii cuttings,weakened the effect of low temperature stress on superoxide dismutase(SOD),peroxidase(POD),catalase(CAT) enzyme activities.Relative electrical conductivity and MDA content of 15 mg · L-1 ABA were the lowest,which were 45.74% and 153.28 nmol · g-1 respectively.Proline content raised by 72.99 μg · g-1 compared with the control.Chilling injury degree of 20 mg · L-1 ABA was significantly lower than other treatments.SOD activity with ABA treatment showed obvious double peak tendency with the prolongation of stress time.POD activity showed the single peak tendency first increasing then decreasing.When ABA content was 15 and 20 mg · L-1,CAT activity showed "decreasing-increasing-decreasing" pattern.