Simultaneous detection and genetic variability of stone fruit viroids in the Czech Republic |
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| Authors: | M Hassan G Gomez V Pallás A Myrta P Rysanek |
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| Institution: | (1) Plant Pathology Division, Faculty of Agriculture, Fayoum University, Fayoum, Egypt;(2) Department of Plant Protection, Faculty of Agrobiology, Food and Natural Resources, Czech University of Agriculture, Prague, Czech Republic;(3) Instituto de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia-CSIC, Avenida de los Naranjos s/n 46022, Valencia, Spain;(4) Certis Europe B.V., Via A. Guaragna, 3-21047 Saronno, VA, Italy |
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| Abstract: | In this paper, we report a large-scale survey for the incidence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) in stone fruit collections and commercial orchards in the Czech Republic. From the 645 samples analysed, PLMVd was
detected in 80 (26.6%) of peaches and the HSVd in 3 (1.3%) of apricot and 1 (0.33%) of peach trees. Sixty-seven accession
of peach (44.6%) from the Czech Clonal GeneBank were infected by PLMVd. In addition, we used naturally infected trees to standardise
the simultaneous detection of PLMVd and HSVd plus host mRNA as the control by means of one-step multiplex RTC-PCR. Eleven
PLMVd and two HSVd isolates were sequenced and analysed. All the PLMVd variants were highly homologous (97–100%) to previously
reported PLMVd variants from Tunisian peach and almond trees, and clustered together in the previously reported phylogenetic
group III. The HSVd variants obtained from apricot and peach trees were included in the previously proposed recombinant group
PH/cit3. |
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| Keywords: | Prunus PLMVd HSVd Multiplex RT-PCR Stone fruit viroids |
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