| 巴西橡胶树抗坏血酸过氧化酶基因HbAPX的克隆及其对水杨酸的应答 |
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| 引用本文： | 罗红丽,闫志烨. 巴西橡胶树抗坏血酸过氧化酶基因HbAPX的克隆及其对水杨酸的应答[J]. 热带生物学报, 2011, 0(3): 197-203 |
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| 作者姓名： | 罗红丽 闫志烨 |
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| 作者单位： | 海南大学海南省热带作物资源可持续利用重点实验室,海南海口570228 |
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| 基金项目： | 海南大学“211工程”热带作物遗传育种与生态保育创新人才培养基金青年教师项目（QNJS-2011-08）;海南省自然科学基金项目（311030）,海南大学“211工程”专项资金（研究生教育教学改革研究项目）资助 |
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| 摘 要： | 在对橡胶树转录组进行测序的基础上,利用模式植物中抗坏血酸过氧化物酶(Ascorbate peroxidase,APX)基因的氨基酸序列进行比对并设计引物,通过RT-PCR方法扩增巴西橡胶树APX基因的cDNA片段,命名为HbAPX.该片段包含1个858核苷酸的开放阅读框,编码285个氨基酸的多肽.生物信息学分析结果表明...
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| 关 键 词： | 巴西橡胶树 抗坏血酸氧化物酶(APX) SA应答 |
Cloning of Ascorbate Peroxidase Gene HbAPX in Hevea brasiliensis and Its Response to Salicylic Acid |
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| LUO Hong-li,YAN Zhi-ye. Cloning of Ascorbate Peroxidase Gene HbAPX in Hevea brasiliensis and Its Response to Salicylic Acid[J]. Journal of Tropical Biology, 2011, 0(3): 197-203 |
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| Authors: | LUO Hong-li YAN Zhi-ye |
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| Affiliation: | (Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Hainan University, Haikou 570228, China) |
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| Abstract: | Based on the sequencing results of RNA-Seq analysis of Hevea brasiliensis and the amino acid se- quence of APX1 ＂in Arabidopsis thaliana, the specific primers were designed and RT-PCR were performed to am- plify the APX cDNA of Hevea brasiliensis（HbAPX）. The results showed that the open reading frame of HbAPX is 858bp encoding 285 amino acids with a conserved ascorbate peroxidase domain ; the amino acid sequence of the HbAPX has 90% homology with APX from Jatropha curcas, and the homology is more than 80% with APX from Populus tomentosa, Gossypium hirsutum, Arabidopsis thaliana, Oryza sativa ; the phylogenetic analysis data indi- cated that HbAPX belong to peroxisome type APX; the quantitative analysis data suggested that the expression of HbAPX was induced by SA, which is probably involved in system acquired resistance and hypersensitivity reac- tion in rubber. |
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| Keywords: | Hevea brasiliensis Ascorbate peroxidase（ APX ） SA response |
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