| 油菜菌核菌拮抗性柑桔内生细菌的筛选及菌种鉴定 |
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| 引用本文： | 罗永兰,张翼,张志元.油菜菌核菌拮抗性柑桔内生细菌的筛选及菌种鉴定[J].热带生物学报,2011(1):26-29. |
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| 作者姓名： | 罗永兰 张翼 张志元 |
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| 作者单位： | [1]长沙学院生物工程与环境科学系,湖南长沙410003 [2]湖南省长沙市植保植检工作站,湖南长沙410013 |
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| 基金项目： | 湖南省教育厅科学研究项目（06C165）; 湖南科技创新团队资助项目 |
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| 摘 要： | 采用对峙培养法,从87个柑桔内生细菌分离物(YS01～YS87)中筛选到对油菜菌核病菌具有拮抗作用的内生细菌分离物6个.结果表明,柑桔内生细菌不同分离物之间对油菜菌核病菌的抑菌作用有显著差异,抑菌能力最强的是YS45,其抑菌圈直径达2.7 cm,显著优于其他分离物.经细菌分类学鉴定,该分离物为枯草芽孢杆菌(Bacill...
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| 关 键 词： | 柑桔内生细菌 油菜菌核病菌 枯草芽孢杆菌 生物防治 |
Screening and Identification of Antagonistic Endophytic Bacteria in Citrus against Sclerotinia sclerotiorum |
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| LUO Yong-lan,ZHANG Yi,ZHANG Zhi-yuan.Screening and Identification of Antagonistic Endophytic Bacteria in Citrus against Sclerotinia sclerotiorum[J].Journal of Tropical Biology,2011(1):26-29. |
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| Authors: | LUO Yong-lan ZHANG Yi ZHANG Zhi-yuan |
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| Institution: | 1.Department of Bioengineering and Environment Science,Changsha University,Changsha 410003,China;2.Plant Protection and Quarantine Station of Changsha,Hunan Province,Changsha 410013,China) |
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| Abstract: | Confronting incubation method has been performed to obtain six antagonistic endophytic bacterial isolates from eighty seven bacterial isolates（YS01-YS87）of the endophytic bacterium in citrus.The results indicated that there were remarkable differences among antagonism against Sclerotinia sclerotiorum of different endophytic bacterial isolates,The inhibitory zone tests showed that the inhibitory activity of the endophytic bacterial isolate YS45 was the greatest in all the tested endophytic bacterial isolates,the inhibiter zone diameter of which was 2.7 cm and significantly bigger than that of others.By systematic bacteriology identification,the isolate was identified to be Bacilus subtilis. |
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| Keywords: | endophytic bacteria in citrus Sclerotinia sclerotiorum Bacillus sublitis biocontrol |
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