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| 水稻锯齿叶矮缩病毒的检测及介体传毒特性 | |
| 引用本文： | 章松柏,宋国威,杨靓,吴祖建,谢联辉. 水稻锯齿叶矮缩病毒的检测及介体传毒特性[J]. 福建农林大学学报(自然科学版), 2013, 42(3) |
| 作者姓名： | 章松柏 宋国威 杨靓 吴祖建 谢联辉 |
| 作者单位： | 1. 福建农林大学植物病毒研究所/福建省植物病毒学重点实验室,福建福州350002;长江大学农学院,湖北荆州434025 2. 福建农林大学植物病毒研究所/福建省植物病毒学重点实验室,福建福州,350002 |
| 基金项目： | 国家973计划项目，国家自然科学基金项目 |
| 摘 要： | 分别建立了水稻锯齿叶矮缩病毒(RRSV)病株的dsRNA基因组检测法和单头介体褐飞虱带毒的RT-PCR法,并结合生物学接种试验,对介体传毒特性进行了初步分析.结果显示:dsRNA基因组鉴定法可以从0.5 g病株样品中快速检测到RRSV,RT-PCR法可以灵敏地应用于褐飞虱带毒、传毒情况的检测;饲毒后褐飞虱成虫、若虫的带毒率分别为75.0%、68.2%,传毒率分别为50.0%、32.5%,说明褐飞虱种群传播RRSV的能力很强,是高度亲和的群体. |
| 关 键 词： | RT-PCR dsRNA基因组检测法 水稻锯齿叶矮缩病毒 褐飞虱 |
Determination of Rice ragged stunt virus and vector transmission characteristics |
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| ZHANG Song-bai , SONG Guo-wei , YANG Liang , WU Zu-jian , XIE Lian-hui. Determination of Rice ragged stunt virus and vector transmission characteristics[J]. Journal of Fujian Agricultural and Forestry University, 2013, 42(3) | |
| Authors: | ZHANG Song-bai SONG Guo-wei YANG Liang WU Zu-jian XIE Lian-hui |
| Abstract: | RT-PCR and viral dsRNA identification assays were established to detect Rice ragged stunt virus(RRSV) in Nilaparvata lugens and rice plants,and vector transmission characteristics were analyzed using artificial inoculation.The results showed that viral dsRNA identification assay could detect virus from rice samples as less as 0.5 g,and RT-PCR assay was suitable for detecting RRSV in individual brown planthopper.The viruliferous ratios of nymphs and adults of N.lugens inoculated by RRSV were 68.2% and 75.0%,respectively.50.0% of nymphs and 32.5% of adults could transmit RRSV after virus acquisition.These results revealed that the brown planthopper population had high compatibility to RRSV with strong capacity to transmit the virus. |
| Keywords: | RT-PCR viral dsRNA identification assay Rice ragged stunt virus Nilaparvata lugens |
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