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| 瓦氏黄颡鱼肝脂酶基因cDNA序列的克隆与序列分析 | |
| 引用本文： | 覃川杰,陈立侨,李二超,杨洁萍,姜雪芹.瓦氏黄颡鱼肝脂酶基因cDNA序列的克隆与序列分析[J].福建农林大学学报(自然科学版),2013,42(3). |
| 作者姓名： | 覃川杰 陈立侨 李二超 杨洁萍 姜雪芹 |
| 作者单位： | 1. 华东师范大学生命科学学院,上海200062;内江师范学院生物系,四川内江641000 2. 华东师范大学生命科学学院,上海,200062 3. 内江师范学院生物系,四川内江,641000 |
| 基金项目： | 国家"973"计划资助项目,国家科技支撑计划课题资助项目,公益性行业(农业)科研专项资助项目,内江师范学院博士基金资助项目 |
| 摘 要： | 利用RT-PCR方法克隆瓦氏黄颡鱼(Pelteobagrus vachelli)肝脂酶cDNA序列片段,并对其基因结构和系统进化关系进行分析.瓦氏黄颡鱼肝脂酶cDNA片段长1065 bp,编码353个氨基酸.肝脂酶氨基酸序列同源性分析结果表明,瓦氏黄颡鱼与其他鱼类的同源性为62%-100%.瓦氏黄颡鱼肝脂酶氨基酸残基包含糖基化位点、催化位点、催化中心三联体位点、脂质结合位点和多肽"盖"等主要结构区域.系统进化树分析表明,瓦氏黄颡鱼肝脂酶与草鱼、鳙、斑马鱼肝脂酶聚为一支.因此,瓦氏黄颡鱼的肝脂酶在鱼类进化中较为保守. |
| 关 键 词： | 肝脂酶 瓦氏黄颡鱼 基因克隆 序列分析 |
Cloning and characterization of cDNA sequence of hepatic lipase gene from darkbarbel catfish Pelteobagrus vachelli |
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| QIN Chuan-jie , CHEN Li-qiao , LI Er-chao , YANG Jie-ping , JIANG Xue-qin.Cloning and characterization of cDNA sequence of hepatic lipase gene from darkbarbel catfish Pelteobagrus vachelli[J].Journal of Fujian Agricultural and Forestry University,2013,42(3). | |
| Authors: | QIN Chuan-jie CHEN Li-qiao LI Er-chao YANG Jie-ping JIANG Xue-qin |
| Abstract: | The partial cDNA sequence of hepatic lipase(HL) in darkbarbel catfish Pelteobagrus vachelli was cloned by RT-PCR method.The obtained HL cDNA was 1065 bp and encoded 343 amino acids.Sequence comparison indicated that the deduced amino acid sequence of P.vachelli hepatic lipase had identity of 62%-100% with that of other fish.The deduced amino acid sequence of P.vachelli hepatic lipase possessed conserved motif of the lipase gene superfamily,included the "lid" sites,potential N-linked glyeosylation sites,catalysis site,catalytie triad and putative lipid binding region.Phylogenetic analysis revealed that the P.vachelli hepatic lipase was closely related to the hepatic lipase in other fish. |
| Keywords: | hepatic lipase Pelteobagrus vachelli gene cloning sequence analysis |
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