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Detection of Rabies Virus Genes by <Emphasis Type="Italic">In-Situ</Emphasis> Polymerase Chain Reaction |
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| Authors: | P Ezhil Praveena R Jayakumar C Balachandran G Thirumurugan G Dhinakar Raj B Murali Manohar |
| Institution: | (1) Indian Veterinary Research Institute, Izatnagar, Bareilly;(2) Department of Veterinary Epidemiology and Preventive Medicine, Chennai;(3) Centralised Clinical Laboratory, Chennai;(4) Department of Animal Biotechnology, Chennai;(5) Department of Veterinary Pathology, Madras Veterinary College, Chennai;(6) Vaccine Research Centre, Madhavaram Milk Colony, Chennai |
| Abstract: | Rabies is diagnosed by FAT in the impression smears of brain tissues. In this study, an attempt was made to diagnose rabies using in situ polymerase chain reaction (ISPCR). A digoxigenin-labelled double-stranded probe specific for a portion of the ‘N’ gene of rabies virus was used. Positive signals were identified as blue dots in the intraneuronal and neuropil areas. |
| Keywords: | in situ PCR rabies diagnosis |
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