8份剑麻种质亲缘关系的ISSR和RAPD分析

为了揭示剑麻栽培品种的遗传多样性，利用ISSR和RAPD分子标记技术对8份剑麻种质的亲缘关系进行分析。结果表明，筛选后选用的8条ISSR引物和8条RAPD引物，分别产生了53条和66条扩增条带，其中多态性条带分别为44条和61条，多态性条带百分率分别为83.02%和92.42%。根据2种标记的扩增结果，用UPGMA法对8份剑麻种质进行聚类分析，供试材料之间具有较高的遗传多样性，其品种间遗传相似系数分别为0.59~0.80和0.52~0.76。2个标记的聚类结果基本一致，但有点差异，可将供试的8份剑麻种质划分为2类群，而且2个标记聚类结果呈显著相关性，相关系数为0.70。可见，剑麻种质资源的遗传多样性丰富。

ISSR and RAPD Analysis of Genetic Relationship in 8 Sisal Germplasm Resources

The objective of this paper is to reveal the genetic diversity of sisal cultivars.The genetic relationships of 8 sisal germplasm resources were analyzed by ISSR and RAPD.Polymorphism was identified by 8 ISSR primers and 8 RAPD primers in the germplasm.53 bands were generated by ISSR with polymorphic bands accounting for 83.02% and similarity coefficient within 0.59 to 0.80.And 66 bands were generated by RAPD with polymorphic bands accounting for 92.42% and similarity coefficient from 0.52 to 0.76.Using cluster analysis（UPCMA） based on those polymorphic bands amplified with ISSR and RAPD primers,the relationships of 8 sisal species were constructed.The clustering results of RAPD and ISSR markers were similar,but had a little difference.The germplasm were divided into 2 clusters,and the significant correlation between ISSR and RAPD markers was observed（r=0.70）.The results revealed that there was rich genetic diversity among resources of sisal.