Development and evaluation of a high-throughput,low-cost genotyping platform based on oligonucleotide microarrays in rice |
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| Authors: | Jeremy D Edwards Jaroslav Janda Megan T Sweeney Ambika B Gaikwad Bin Liu Hei Leung David W Galbraith |
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| Institution: | (1) University of Florida, Gulf Coast Research & Education Center, Wimauma, FL 33598, USA;(2) Department of Plant Sciences and Bio5 Institute for Collaborative Bioresearch, University of Arizona, Tucson, AZ 85721, USA;(3) National Research Centre on DNA Fingerprinting, National Bureau of Plant Genetic Resources, New Delhi, India;(4) Guangdong Academy of Agricultural Sciences (GDAAS), Guangdong, Pr China;(5) International Rice Research Institute (IRRI), Los Banos, The Philippines |
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| Abstract: | Background We report the development of a microarray platform for rapid and cost-effective genetic mapping, and its evaluation using
rice as a model. In contrast to methods employing whole-genome tiling microarrays for genotyping, our method is based on low-cost
spotted microarray production, focusing only on known polymorphic features. |
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