功能大豆寡肽蛋白饲料的研制及其固态发酵工艺参数的优化研究

本文就多菌种组合协同固态发酵生产功能大豆寡肽蛋白饲料工艺参数优化的工业化研究结果进行了总结。固态开放式生产功能大豆寡肽蛋白饲料的模式为:多菌种组合→液态接种→深层(≥10cm)、堆式、开放式发酵。经筛选的复合菌系为:乳酸菌、枯草芽孢杆菌、粪链球菌、黑曲霉与酵母菌。经优化的发酵工艺参数为:时间48h;pH值为6.0~8.0;24h前温度30±1℃,24h后温度28±1℃;菌液接种量5%(v/w);料水比1∶0.7;底物组成:豆粕70%、麸皮27%、玉米粉3%。并就影响因素中最为关键的菌种因素与烘干条件进行了详细讨论。

The Study on Optimized Technical Parameters of Solid State Fermentation Soybean Meal with Associative Muti-bacterial Species to Produce Functional Soy Oiigopeptide-protein Feeds

This paper summarized the industrialized results of optimized technical parameters of solid state fermentation soybean meal with associative muti-bacterial species to produce functional soy oligopeptide-protein feeds.The model is：（Type of bacterial species） associative muti-bacterial species→（Inoculation manner） liquid state inoculation→（Type of technics） deep level（≥10cm）,heap,open-style fermentation.Screened complex muti-bacterial species are： Lactobacillus,Bacillus subtilis,Streptococcus faecalis,Aspergillus niger and Saccharomycetes.Optimized technical parameters are： Time,48 h;pH 6.0 -8.0;Temperature,before 24h 30 ±1℃ and then 28 ±1℃;Inoculated volume of complex muti-bacterial species： 5%（v/w）;Feed/water： 1∶0.7;Components of the substrate： soybean meal 70%,bran 27%,maiz 3%.And the key factors of bacterial species and the conditions of oven drying were also discussed in detail.