镜鲤头长及头长体长比性状的主效QTL挖掘

为挖掘镜鲤头长及头长体长比性状的主效QTL区间,实验利用368个SSR、336个SNP标记对镜鲤良种后代杂交F1群体的68个个体进行基因型检测,运用JoinMap 4.0软件包构建遗传连锁图谱。该图谱包含535个分子标记并被分配到50个连锁群上,覆盖基因组总长度为2 244.66 cM,标记间平均距离为4.63 cM。利用MapQTL 5.0(interval mapping,IM)区间作图法进行QTL检测。结果显示,共得到2个与头长相关的QTL区间,分别分布在LG21和LG42,可解释型变异分别为28.2%、32.6%;6个与头长体长比性状相关的QTL位于LG8、LG15、LG18、LG21、LG39、LG40,可解释表型变异范围是16.4%~49.3%。全部QTL区间中贡献率大于20%的主效QTL有7个,HL-21和HL-42是头长性状的主效区间;HBR-8、HBR-15、HBR-21、HBR-39和HBR-40是头长体长比性状的主效QTL区间。利用SPSS的一般线性模型(GLM)针对另一群体进行验证,结果表明HLJ692与镜鲤头长体长比显著相关。

Excavating the main effect of quantitative trait loci related to head length, head length and body length ratio in mirror carp(Cyprinus carpio)

In this study,the purpose is to excavate the main effect of quantitative trait loci related to head length,head length and body length ratio in mirror carp(Cyprinus carpio L.).A group of F₁ hybrids common carp including 68 individuals was used to construct a linkage map using 535 markers,which were arranged to 50 groups covering 2 244.66 cM genetic distance with an average space of 4.63 cM.MapQTL5.0 software was conducted to detect the quantitative trait loci(QTL)for HL and HBR using interval mapping(IM).Results showed that two QTLs were identified for head length on the linkage groups of LG21(SNP1392-HLJ643),LG42(SNP0562-SNP0109),which explained 14.9% to 32.6% of the total variation of the head length;six QTLs were responsible for head length and body length ratio associated with the linkage groups of LG8(HLJ423),LG15(SNP0002),LG18(HLJ1042-SNP0046),LG21(SNP1392-SNP0818),LG39(HLJ322),LG40(SNP0026),which explained 16.4% to 49.3% of the phenotypic variations.Seven QTLs explaining above 20%,HL-21 and HL-42 were major QTLs associated to the head length of common carp;HBR-8,HBR-15,HBR-21,HBR-39 and HBR-40 were major QTLs associated to the head length and body length ratio.These results indicated that it was highly probable that these QTLs were the main regions of the head length,head length and body length ratio development.SPSS general linear model(GLM)verified another group,and results showed that the HLJ692 was significant correlation for mirror carp head length and body length ratio(P<0.05).