| 鹅IGF-Ⅰ基因5′调控区单核苷酸多态性分析 |
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| 引用本文： | 杜晓东,张绍胜,姜润深,夏生林,耿照玉,袁绍友.鹅IGF-Ⅰ基因5′调控区单核苷酸多态性分析[J].畜牧与饲料科学,2009(2). |
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| 作者姓名： | 杜晓东 张绍胜 姜润深 夏生林 耿照玉 袁绍友 |
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| 作者单位： | [1]安徽农业大学动物科技学院,安徽合肥230036 [2]安徽省芜湖市动物疾控中心,安徽芜湖241000 [3]安徽省皖西白鹅原种场,安徽六安237005 |
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| 摘 要： | 以皖西白鹅和朗德鹅为素材,设计引物对鹅的IGF-Ⅰ基因5′端非编码区序列克隆测序,运用PCR-SSCP方法检测IGF-Ⅰ基因5′调控区的单核苷酸多态性。检测发现该区域存在4个SNP位点,分别是：A-26T、A-215G、A-314G、A-325T。
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| 关 键 词： | 鹅 IGF-Ⅰ基因 PCR-SSCP 单核苷酸多态性 调控区 |
Polymorphism Analysis on the Single Nucleotide in the 5' Regulatory Region of IGF-1 Gene in Goose |
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| DU Xiao-dong,et al.Polymorphism Analysis on the Single Nucleotide in the 5' Regulatory Region of IGF-1 Gene in Goose[J].Animal Husbandry and Feed Science,2009(2). |
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| Authors: | DU Xiao-dong |
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| Institution: | College of Animal Science and Technology;Anhui Agricultural University;Hefei 230036;China |
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| Abstract: | With Wanxi white geese and Landes geese as materials,the primers were designed for cloning and sequencing the sequence in 5' non-coding region of IGF-1 gene in goose. The polymorphism of single nucleotide in 5' regulatory region of IGF-1 gene was detected by PCR-SSCP method. It was found through detection that there were 4 SNP sites in the region and they were A-26T,A-215G,A-314G and A-325T resp. |
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| Keywords: | goose IGF-1gene PCR-SSCP single nucleotide polymorphisms regulating region |
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