4种植物提取物对茶炭疽病菌的体外抑制作用

以丙酮作溶剂对芦柑皮、艾蒿、香樟叶和侧柏叶进行浸提;采用菌丝生长速率法测定各提取物对茶炭疽病菌菌丝的抑制作用,采用水琼脂培养法测定提取物对分生孢子萌发的抑制效果。结果表明:1 500μg/mL浓度下,各提取物对茶炭疽病菌菌丝均有抑制作用,其中芦柑皮96h的抑制率达到93.32%,艾蒿、香樟叶和侧柏叶96h的抑制率分别为61.10%、55.64%和48.20%,EC50分别为8.981、839.575、1 058.682μg/mL和1 429.094μg/mL;芦柑皮、艾蒿、香樟叶和侧柏叶提取物对分生孢子的抑制率分别为34.85%、85.54%、55.40%和59.68%,EC50分别为5 764.142、281.121、1 502.49μg/mL和969.369μg/mL。芦柑皮、艾蒿丙酮提取物具有开发植物源杀菌剂的潜力。

Inhibition of 4 plant extracts against Gloeosporium theae-sinesis in vitro

This study was to test the inhibitory effects of 4 plant extracts on Gloeosporium theae-sinesis Miyake in vitro.Citrus madurensis peels,Artemisia argyi plant,Cinnamomum camphora leaves and Platycladus orientalis leaves were extracted by acetone;Mycelium growth rate methods and water agar culture were applied to determine the inhibitory effects of these extracts against mycelium growth and spore germination of G.theae-sinesis,respectively.All extracts had effects on mycelium growth.After treatment for 96 h,the inhibition rates of C.madurensis peels,A.argyi plant,C.camphora leaves and P.orientalis leaves with dosage of 1 500 μg/mL were 93.32%,61.10%,55.64% and 48.20%,respectively,while their EC50 values were 8.981 μg/mL,839.575 μg/mL,1 058.682 μg/mL and 1 429.094 μg/mL,respectively;Their inhibition rates against spore germination were 34.85%,85.54%,55.40% and 59.68%,respectively,and EC50 values were 5 764.142,281.121,1 502.49 μg/mL and 969.369 μg/mL,respectively.The acetone extracts of C.madurensis peels and A.argyi plant had great potential for the development of botanical fungicides.