小麦种质N9820抗白粉病的特异基因表达谱分析

小麦白粉病是由白粉菌(Blumeria graminis f.sp.tritici)引起的世界性真菌病害之一,白粉病原菌的生理小种多,毒性变异速度快,很容易造成品种抗病性的丧失。培育抗病新品种有两种基本措施,一是发现与利用新的抗病基因;二是诱导防御

Expression of special genes resistant to powdery mildew ( Blumeria graminis f.sp.tritici) in wheat germplasm N9820

Wheat germplasm N9820,developed by our research group,is a resistant material to powdery mildew.In order to understand the resistant mechanism of wheat germplasm N9820 to powdery mildew infection,a suppression subtraction hybridization(SSH) cDNA library was constructed with cDNA from N9820 leaf inoculated with Blumeria graminis as the tester and cDNA from N9820 healthy leaf as the driver.A total of 122 positive clones were randomly chosen from the SSH-cDNA library.After screening of repeated and redundant sequences,61 ESTs were acquired.Nucleic acid and protein homology search were performed using the BLAST(Basic Local Alignment Search Tool) program with the default settings at NCBI website(http://www.ncbi.nlm.nih.gov).BlastX results in nr-protein database revealed that 35 ESTs were highly homologous with known proteins involved in signal transduction,metabolism,cell structure,energy metabolism,transport,protein synthesis and processing,and disease resistance.BlastNr results showed that 47 ESTs had high identities with known ESTs,and 14 ESTs matched none in the nr-database.Compared with BlastX and BlastNr analysis,19 ESTs were both in the nucleic acid and protein databases including 5 for energy metabolism,2 for transport,3 for protein synthesis and processing,and 2 for disease resistance.The most frequent sequence was ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit.