中性电解水对鸡蛋表面的清洗灭菌效果

为寻求一种高效、安全、无污染的禽蛋清洗消毒剂，采用无隔膜电解装置电解稀盐酸溶液制备中性电解水（pH值6.0～7.5）考查不同有效氯浓度、处理时间和温度条件下中性电解水对鸡蛋人工接种鸡白痢沙门氏菌（Salmonella pullorum，鸡蛋表面的初始菌落数对数为6.19～6.26 log10 (cfu/g)）和大肠杆菌O157:H7（鸡蛋表面的初始菌落数对数为6.12～6.19 log10 (cfu/g)）的杀灭效果。结果表明，中性电解水对2种病菌均具有较强的杀灭效果，其杀菌效果随着有效氯浓度和处理时间的增加而增强，但温度对中性电解水的杀菌效果影响不显著。对菌悬液的杀菌试验表明：当中性电解水有效氯质量浓度为1.5 mg/L时，可以在20℃下3 min内完全杀灭鸡白痢沙门氏菌（初始含菌数的对数为 8.12 log10 (cfu/mL)）；质量浓度为2 mg/L时，可以100%杀灭大肠杆菌O157:H7（初始含菌数的对数为7.78 log10 (cfu/mL)）。当中性电解水清洗消毒被人工污染的鸡蛋表面时，有效氯质量浓度为12 mg/L、处理3 min可将鸡蛋表面的鸡白痢沙门氏菌全部杀灭，大肠杆菌O157:H7菌落数对数降低到1.0 log10 (cfu/g) 以下，且处理废液中没有残存菌，无二次污染问题。因此，中性电解水可以代替化学杀菌剂应用于鸡蛋清洗消毒。

Disinfection effect of neutral electrolyzed water for shell egg washing

For the sake of developing an effective, safe and no pollution disinfectant to reduce or eliminate food-borne pathogens on shell eggs, the bactericidal activity of neutral electrolyzed water (NEW, pH 6.0-7.5) generated by electrolysis of diluted hydrochloric acid in a non-membrane electrolytic cell for inactivation of Salmonella pullorum, Escherichia coli O157:H7 and artificial inoculated shell eggs (initial logarithm of population of two pathogens on the eggs were approximately 6.19-6.26 log10 (cfu/g) and 6.12-6.19 log10 (cfu/g) respectively) was evaluated. The bactericidal activity of NEW for both of pathogens increased with increasing the available chlorine concentration and treatment time. A 100% inactivation of S. pullorum (initial logarithm of population of pathogen 8.12 log10 (cfu/mL)) and E. coli O157:H7 (initial logarithm of population of pathogen 7.78 log10 (cfu/mL)) was resulted in NEW with available chlorine more than 1.5 and 2 mg/L for 3 min at 20oC, respectively. Temperature had no markedly effect on the bactericidal activity of neutral electrolyzed water. The S. pullorum on the surface of shell eggs was completely killed and E. coli O157:H7 logarithm of counts decreased to less than 1.0 log10 (cfu/g) by NEW containing 12 mg/L available chlorine for 3 min without viable cells in the washing water after treatment. Results indicate that NEW has a great potential to be used as effective disinfectant agent for egg washing.