东方田鼠组织与血清中抗日本血吸虫相关蛋白的分离与筛选

本试验以兔、牛、人及感染日本血吸虫后小鼠血清蛋白为对照,分别提取东方田鼠和小鼠的心脏、肝脏、脾脏、肺脏、肾脏、肌肉、皮肤、骨髓和脑组织蛋白,进行聚丙烯酰胺凝胶电泳,综合比较和筛选东方田鼠组织及血清中潜在的抗日本血吸虫相关蛋白组分。结果表明,随着感染日龄的增加,小鼠在感染日本血吸虫后12和45 d,其血清中85 ku附近蛋白质条带较东方田鼠表达量明显上调,且有继续升高的趋势;东方田鼠肝脏及血清中150 ku组分蛋白电泳条带与其他动物相同组织中相应蛋白质在表达量存在显著差异,蛋白质表达丰度高,该蛋白质组分可能与东方田鼠抗日本血吸虫作用相关。

Separation and Screening Proteins Associated with Resistant to Schistosoma japonicum Infection from Tissues and Serum of Microtus fortis

In order to comprehensive compare and screen potential proteins resistance to Schistosoma japonicum infection from tissues and serum of Microtus fortis, the heart, liver, spleen, lung, kidney, muscle, skin, bone marrow, brain tissue and serum protein of Microtus fortis were collected respectively. The same proteins obtained from rabbits, cattle and mice infected with Schistosoma japonicum as control. The individual soluble proteins were separated by SDS-PAGE and stained with kaomasi blue R-250. The band sizes and their differences were analyzed. Results showed that the 85 ku proteins fractions in serum was more obviously and highly expressed after 12, 45 days post-infection in mice than in Microtus fortis; the 135 ku proteins fractions in liver and serum of Microtus fortis was characterized and more highly expressed than other animals. It maybe an important effective molecule against Schistosoma japonicum infection in Microtus fortis.