| 棉花MADS-box蛋白基因（GhMADS-13）的克隆和表达分析 |
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| 引用本文： | 吴东,喻树迅,范术丽,宋美珍,王力娜.棉花MADS-box蛋白基因（GhMADS-13）的克隆和表达分析[J].广西农业生物科学,2009(2):223-228. |
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| 作者姓名： | 吴东 喻树迅 范术丽 宋美珍 王力娜 |
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| 作者单位： | 中国农业科学院棉花研究所;农业部棉花遗传改良重点实验室; |
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| 基金项目： | 863计划项目(2006AA10A109);;973计划项目(2004CB117306)资助 |
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| 摘 要： | 一组具有MADS-box结构域的转录因子在控制花器官的诱导与发育中起着重要作，以中棉所36（CCRI）均一化全长cDNA文库为基础，结合EST测序分析，分离获得一个棉花的MADS-box基因全长cDNA。该基因cDNA全长1079bp，包含一个732bp的完整的开放阅读框，编码234个氨基酸，具有典型的MADS-box结构域和完整的K区，命名为GhMADS-13（GenBank：FJ409870）。与拟南芥、葡萄等植物的AGL6类基因具有高度的同源性。实时定量RT-PCR分析表明，GhMADS-13在CCRI36的花器官发育中早期表达量逐步增加，后期有下降趋势。推测GhMADS-13可能在开花诱导和花器官发育过程中起着重要作用。
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| 关 键 词： | 棉花 MADS-box基因 表达分析 |
Cloning and Expression Analysis of a MADS-box Protein Gene(GhMADS-13) from Upland Cotton |
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| Wu Dong Yu Shuxun Fan Shuli Song Meizhen Wang Li'na Cotton Research Institute of Chinese Academy of Agricultural Sciences,Key Laboratory of Cotton Genetic Improvement,Ministry of Agriculture,Anyang.Cloning and Expression Analysis of a MADS-box Protein Gene(GhMADS-13) from Upland Cotton[J].Journal of Guangxi Agricultural and Biological Science,2009(2):223-228. |
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| Authors: | Wu Dong Yu Shuxun Fan Shuli Song Meizhen Wang Li'na Cotton Research Institute of Chinese Academy of Agricultural Sciences Key Laboratory of Cotton Genetic Improvement Ministry of Agriculture Anyang |
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| Institution: | Wu Dong Yu Shuxun Fan Shuli Song Meizhen Wang Li'na Cotton Research Institute of Chinese Academy of Agricultural Sciences,Key Laboratory of Cotton Genetic Improvement,Ministry of Agriculture,Anyang,455000 |
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| Abstract: | A group of regulatory factors containing the MADS-box domain plays an important role in controlling floral organ formation and development. A new cotton flowering-related MADS-box gene cDNA was isolated named as GhMADS-13(GenBank accession number:FJ409870) on this basis of a normalized full-length cDNA library of CCRI 36 combination with the sequencing and analysis of expressed sequence tags(ESTs). The fragment was 1 079 bp long and contained an open reading frame of 732 bp coding a polypeptide of 234 amino... |
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| Keywords: | Upland cotton （Gossypium hirsutum L ） MADS-box gene Expression analysis |
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