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| 组织培养中不同抗生素对南方红豆杉内生菌抑制研究 | |
| 引用本文： | 秦宇,熊兴耀,张家银. 组织培养中不同抗生素对南方红豆杉内生菌抑制研究[J]. 湖南农业科学, 2012, 0(9): 13-15 |
| 作者姓名： | 秦宇 熊兴耀 张家银 |
| 作者单位： | 1. 湖南农业大学园艺园林学院,湖南 长沙,410128 2. 湖南农业大学园艺园林学院,湖南 长沙 410128;中国农业科学院蔬菜花卉研究所,北京100081;湖南省作物种质创新与资源利用重点实验室,湖南 长沙 410128 3. 国家中医药管理局亚健康干预技术实验室,湖南 长沙,410128 |
| 摘 要： | 抗生素能有效抑制组织材料中内生菌,但红豆杉在组织培养过程中易褐化,从而影响愈伤组织诱导。试验通过分析不同类型、不同浓度抗生素对南方红豆杉组织培养的内生菌的抑制作用,找出南方红豆杉内生菌抑制的最适抗生素及其最适浓度。试验结果表明:500 mg/L氟康唑抑制效果最好,且组织培养过程中红豆杉组织茎段不发生褐化。 |
| 关 键 词： | 南方红豆杉 内生菌 抗生素 组织培养 |
Inhibitory Effects of Different Antibiotics on Endophyte in Taxus chinensis var.mairei |
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| QIN Yu , XIONG Xing-yao , ZHANG Jia-yin. Inhibitory Effects of Different Antibiotics on Endophyte in Taxus chinensis var.mairei[J]. Hunan Agricultural Sciences, 2012, 0(9): 13-15 | |
| Authors: | QIN Yu XIONG Xing-yao ZHANG Jia-yin |
| Affiliation: | 1.College of Horticulture and Landscape,HNAU,Changsha 410128,PRC;2.Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,PRC;3.Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop,Changsha 410128,PRC;4.State Administration of Traditional Chinese Medicine Sub-health Intervention Technology Laboratory,Changsha 410128,PRC) |
| Abstract: | Antibiotics can effectively inhibit the endophyte in tissue materials,but because of the easy browning during tissue culture of Taxus chinensis var.mairei,thereby affecting callus induction.The inhibitory effects of different types and concentrations of antibiotics on endophyte of Taxus chinensis var.mairei were analyzed in order to find the optimum antibiotics and concentration for inhibition of endophyte of Taxus chinensis var.mairei.The results showed that 500 mg/L of fluconazole had good inhibitory effect,and the stem of Taxus chinensis var.mairei did not turn brown. |
| Keywords: | Taxus chinensis var.mairei endophyte antibiotics tissue culture |
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