| 黄瓜无苦味基因bi的分子标记研究 |
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| 引用本文： | 池秀蓉,顾兴芳,张圣平,王晓武,王烨.黄瓜无苦味基因bi的分子标记研究[J].园艺学报,2007,34(5):1177-1182. |
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| 作者姓名： | 池秀蓉 顾兴芳 张圣平 王晓武 王烨 |
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| 作者单位： | （中国农业科学院蔬菜花卉研究所，北京100081） |
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| 摘 要： | 以黄瓜纯合自交系9110Gt（bibi）和03828(BiBi)为双亲获得的F2代为试材，利用分离群体组群分析法，从256对AFLP选择性引物中获得了与营养器官无苦味位点连锁距离为6.43 cM的AFLP分子标记TG/GCT150，并成功地将其转化为操作简便、表现稳定的SCAR标记SC87。
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| 关 键 词： | 黄瓜 苦味 分子标记 AFLP SCAR |
| 文章编号： | 0513-353X（2007）05-1177-06 |
| 修稿时间： | 2007-05-22 |
Identification of Molecular Markers Linked to Foliage Non-bitterness Gene(bi)in Cucumis sativus L. |
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| CHI Xiu-rong,GU Xing-fang,ZHANG Sheng-ping,WANG Xiao-wu,WANG Ye.Identification of Molecular Markers Linked to Foliage Non-bitterness Gene(bi)in Cucumis sativus L.[J].Acta Horticulturae Sinica,2007,34(5):1177-1182. |
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| Authors: | CHI Xiu-rong GU Xing-fang ZHANG Sheng-ping WANG Xiao-wu WANG Ye |
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| Institution: | (Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China) |
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| Abstract: | In this study, AFLP technique and the method of bulked segregant analysis were used to study the F2 progeny of purified cucumber line 9110Gt（bibi）×03828（BiBi）.One AFLP molecular markers TG/GCT150 linked to non-bitterness gene in cucumber with a genetic distance 6.43 cM were identified from 256 primer combinations. This band was collected and sequenced to synthesize a sequence characterized amplified region (SCAR) primer, and TG/GCT150 was converted to SCAR marker SC87 successfully. |
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| Keywords: | AFLP SCAR |
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