| 中国葡萄属野生种抗旱基因的分子标记及遗传分析 |
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| 引用本文： | 杨亚州,王跃进,张剑侠,张朝红.中国葡萄属野生种抗旱基因的分子标记及遗传分析[J].园艺学报,2007,34(5):1087-1092. |
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| 作者姓名： | 杨亚州 王跃进 张剑侠 张朝红 |
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| 作者单位： | (西北农林科技大学园艺学院, 农业部西北园艺植物种质资源与遗传改良重点开放实验室, 陕西省农业分子生物学重点实验室, 陕西杨凌 712100) |
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| 基金项目： | 教育部跨世纪优秀人才培养计划 |
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| 摘 要： | 以葡萄属种间杂交组合燕山葡萄×河岸葡萄的F_1代群体为试材,采用BSA法构建抗旱植株DNA混合样,从300个随机引物中初步筛选出7个可以扩增出多态性DNA的随机引物,用于对供试材料扩增分析,获得了与中国葡萄属野生种抗旱基因连锁的RAPD标记S226-1100、S271-550、S1165-1500、S264- 1300、S195-1000、S1345-1400和S513-1700。并将7个RAPD标记在中国葡萄属野生种、欧洲葡萄、美洲种的冬葡萄和沙地葡萄等18个株系或品种中做了进一步分析。对与目标性状连锁较紧密的RAPD标记S226- 1100进行了克隆测序,转化成专一性的SCAR标记DR-760。经JoinMap 3.0软件分析表明,RAPD标记S226-1100、S271-550、S1165-1500与目标基因位点处于同一个连锁群上,其中以S226-1100与目标基因位点距离较近,为21.8cM。
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| 关 键 词： | 葡萄属 中国野生种 抗旱性 RAPD SCAR |
| 文章编号： | 0513-353X（2007）05-1087-06 |
| 修稿时间： | 2006-12-22 |
Molecular Markers Linked to Drought Resistance Gene in Chinese Wild Vitis Species and Their Genetic Analysis |
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| YANG YA-zhou,WANG Yue-jin,ZHANG Jian-xia,ZHANG Chao-hong.Molecular Markers Linked to Drought Resistance Gene in Chinese Wild Vitis Species and Their Genetic Analysis[J].Acta Horticulturae Sinica,2007,34(5):1087-1092. |
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| Authors: | YANG YA-zhou WANG Yue-jin ZHANG Jian-xia ZHANG Chao-hong |
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| Institution: | (College of Horticulture, Northwest A and F University, Key Laboratory of Northwest Horticulture Plant Germplasm and Genetic Improvement of Ministry of Agriculture of the People's Republic of China, Shaanxi Provincial of Molecular Biology for Agriculture, Yangling, shaanxi 712100, China) |
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| Abstract: | Random amplified polymorphic DNA(RAPD)and bulked segregant analysis(BSA)were employed to detect molecular marker linked to drought resistance gene in the wild grapes(Vitis L.)native to China. DNA pools related to drought resistance were consisted by using the F_1 progenies of V. yeshanensis×V.riparia. Seven RAPD markers,S226-1100,S271-550, S1165-1500, S264-1300, S195-1000, S1345- 1400 and S513-1700, were obtained and seven primers were screened from 300 random primers. Then the 7 markers were further investigated in a set of 18 genotypes of Chinese wild Vitis species, V. vinifera, V. ber- landieri, and V. rupestris. Additionally, RAPD fragment S226-1100 was cloned and sequenced, then conver- ted into SCAR maker DR-760. Genetic analysis by JoinMap 3.0 showed that RAPD markers S226-1100, S271-550 and S1165-1500 were linked to the drought resistance gene. The linking distance between RAPD marker S226-1100 and the gene locus was 21.8 cM. |
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| Keywords: | RAPD SCAR |
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