| 3种禽类呼肠孤病毒血清学相关性及致细胞病变差异分析 |
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| 引用本文： | 陈仕龙,陈少莺,程晓霞,江斌,林锋强,王劭,朱小丽,李兆龙,张世忠.3种禽类呼肠孤病毒血清学相关性及致细胞病变差异分析[J].畜牧兽医学报,2011,42(4). |
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| 作者姓名： | 陈仕龙 陈少莺 程晓霞 江斌 林锋强 王劭 朱小丽 李兆龙 张世忠 |
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| 作者单位： | 福建省农业科学院畜牧兽医研究所,福州,350013;福建省畜禽疫病防治工程技术研究中心,福州,350013 |
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| 基金项目： | 福建省公益类项目(2010R1101003-5); 福建省农科院创新团队项目(STIF-Y02) |
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| 摘 要： | 本研究旨在研究3种不同疾病型禽类呼肠孤病毒间的抗原性关系及病毒的培养特性。作者通过血清中和试验测定了禽呼肠孤病毒(ARV S1133株)、番鸭呼肠孤病毒(MDRV 9710株)、新型鸭呼肠孤病毒(NDRV NP01株)3种禽类呼肠孤病毒的血清学相关性,统计抗原相关性R值;并应用部分禽胚原代细胞及哺乳动物传代细胞对这3种病毒的培养特性进行了初步研究。结果表明,3种病毒株之间的R值很小,抗原相关性较低;三者具有广泛的细胞亲嗜性,能在多种细胞中增殖,并产生细胞病变,但病毒致细胞病变特征有所差异,ARV和NDRV均以巨融合为主,而MDRV则以细胞圆缩坏死为主。上述结果表明导致禽类不同疾病的ARV、MDRV和NDRV三者之间的抗原相关性较低,病毒的细胞培养特性也不同,细胞病变类型的差别提供了一种初步鉴别禽类呼肠孤病毒的方法。
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| 关 键 词： | 禽呼肠孤病毒 番鸭呼肠孤病毒 新型鸭呼肠孤病毒 中和试验 抗原性变异 细胞病变 |
The Comparison of Serologic Relativity and CPE Types of 3 Avian Reovirus Strains Induced Different Diseases |
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| CHEN Shi-long,CHEN Shao-ying,CHENG Xiao-xia,JIANG Bin,LIN Feng-qiang,WANG Shao,ZHU Xiao-li,LI Zhao-long,ZHANG Shi-zhong.The Comparison of Serologic Relativity and CPE Types of 3 Avian Reovirus Strains Induced Different Diseases[J].Acta Veterinaria et Zootechnica Sinica,2011,42(4). |
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| Authors: | CHEN Shi-long CHEN Shao-ying CHENG Xiao-xia JIANG Bin LIN Feng-qiang WANG Shao ZHU Xiao-li LI Zhao-long ZHANG Shi-zhong |
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| Institution: | CHEN Shi-long1,2,CHEN Shao-ying1,CHENG Xiao-xia1,JIANG Bin1,LIN Feng-qiang1,WANG Shao1,ZHU Xiao-li1,LI Zhao-long1,ZHANG Shi-zhong1,2 (1.Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of AgriculturalSciences,Fuzhou 350013,China,2.Fujian Animal Diseases Control Technology DevelopmentCenter,China) |
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| Abstract: | This study was conducted to compare the antigenic relativity and cytopathic effect(CPE) characteristics of 3 avian reovirus isolates induced different diseases.The serologic relatedness of avian reovirus(ARV) S1133 strain,muscovyduck reovirus(MDRV) MW9710 strain,novel duck reovirus(NDRV) NP01 strain were determined using cross virus-neutralization(VN) tests.Two avian and five mammalian cell lines were evaluated for their application in propagating these avian reovirus strains.Relatedness(R) values,determine... |
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| Keywords: | avian reovirus muscovy duck reovirus novel duck reovirus virus neutralization test antigenic variation cytopathogenic effect |
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