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| 藏系绵羊KAP3.2基因多态性及其对部分经济性状的影响 | |
| 引用本文： | 王志有,陈玉林,徐秋良,马忠仁,祁全青. 藏系绵羊KAP3.2基因多态性及其对部分经济性状的影响[J]. 畜牧兽医学报, 2011, 42(2) |
| 作者姓名： | 王志有 陈玉林 徐秋良 马忠仁 祁全青 |
| 作者单位： | 1. 青海大学农牧学院,西宁,810016 2. 西北农林科技大学动物科技学院,杨凌,712100 3. 郑州牧业工程高等专科学校,郑州,450011 4. 西北民族大学,兰州,730030 5. 青海省三角城种羊场,刚察,812300 |
| 基金项目： | 农业公益性行业科研专项“毛用羊现代产业技术研究与示范” |
| 摘 要： | 采用PCR-SSCP和DNA测序技术,对高原型、欧拉型和乔科型3个类型共254只藏绵羊KAP3.2基因的部分序列进行多态性分析,并利用最小二乘线性模型研究了其多态性与体质量、毛长和产毛量性状的关联性。结果,3个群体藏绵羊在KAP3.2基因座上均检测到AA、AB和BB 3种基因型,高原型以A等位基因为主,欧拉型和乔科型以B等位基因为主。3个群体基因型频率均处于Hardy-Weinberg非平衡状态,高原型和欧拉型达中度多态,乔科型为低度多态。测序表明KAP3.2基因271 bp处存在1个C→T的突变,属同义突变。体质量性状上,3类藏绵羊各基因型间均差异不显著;毛长性状上,3类藏绵羊AA基因型均显著高于BB型(P<0.05或P<0.01),而与AB型间无显著差异;产毛量性状上,3类藏绵羊AA基因型均显著高于BB和AB型(P<0.05)。结果表明,KAP3.2基因可作为影响藏系绵羊毛长和产毛量性状的分子标记。 |
| 关 键 词： | 藏系绵羊 PCR-SSCP KAP3.2基因 分子标记 |
Polymorphism of KAP3.2 Gene and Its Effect on Partial Economic Traits in Tibetan Sheep |
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| WANG Zhi-you,CHEN Yu-lin,XU Qiu-liang,MA Zhong-ren,QI Quan-qing. Polymorphism of KAP3.2 Gene and Its Effect on Partial Economic Traits in Tibetan Sheep[J]. Chinese Journal of Animal and Veterinary Sciences, 2011, 42(2) | |
| Authors: | WANG Zhi-you CHEN Yu-lin XU Qiu-liang MA Zhong-ren QI Quan-qing |
| Affiliation: | WANG Zhi-you1,CHEN Yu-lin2,XU Qiu-liang3,MA Zhong-ren4,QI Quan-qing5 (1.Agriculture and Animal Husbandry College,Qinghai University,Xining 810016,China,2.College of Animal Science and Technology,Northwest A&F University,Yangling 712100,3.Zhengzhou College of Animal Husbandry Engineering,Zhengzhou 450011,4.Northwest University for Nationalities,Lanzhou 730030,5.SanjiaochengSheep Breeding Farm of Qinghai Province,Gangcha 812300,China) |
| Abstract: | PCR-SSCP and DNA sequencing techniques were used to analyze the polymorphism of partial sequence of KAP3.2 gene in 254 Tibetan sheep including three types(Plateau,Oula and Qiaoke).Based on the data,the correlation between the polymorphisms and production traits(body weight,wool length and wool yield) was studied using least square linear model.The results showed that three genotypes(AA,AB and BB) were detected at KAP3.2 gene locus.Allele A was predominant in Plateau type,and allele B was predominant in Oula... |
| Keywords: | tibetan sheep PCR-SSCP KAP3.2 gene molecular marker |
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