乳杆菌和双歧杆菌对碳水化合物体外利用的比较研究

在含有13种碳水化合物(包括低聚果糖(fructooligosaccharide,FOS)、FOS(Vilof)、低聚木糖、低聚半乳糖、短链菊粉、长链菊粉、低聚异麦芽糖、水苏糖、抗性糊精、聚葡萄糖、L-阿拉伯糖、乳糖醇和赤藓糖醇)作为唯一碳源的纯培养基上,比较12株乳杆菌和6株双歧杆菌的生长情况,记录培养24?h和72...

Comparison of in Vitro Carbohydrate Utilization by Lactobacilli and Bifidobacteria

The growth of twelve Lactobacillus strains and six Bifidobacterium strains on a medium containing 13 carbohydrates including prebiotics (fructooligosaccharide (FOS), FOS (Vilof), xylooligosaccharide (XOS), galactooligosaccharide (GOS), short-chain inulin, long-chain inulin, isomalto oligosaccharide (IMO), stachyose, resistant dextrin, polydextrose, L-arabinose, lactitol, and erythritol) as sole carbon sources was compared using lactose as a control. Optical density at 600 nm (OD600 nm) and pH were recorded after 24 and 72 h of culture. Results showed great differences in the growth of strains on different carbohydrates. Resistant dextrin, polydextrose, and erythritol showed very limited growth promotion effects on all the strains after 24 h, probably because these strains lack the enzymes that hydrolyze glycosidic bonds. The growth promotion effects of FOS, XOS, GOS, inulin, stachyose, L-arabinose and lactitol were strain specific, presumably because of the different extracellular or intracellular enzymes secreted by each strain. Long-chain inulin slowed down the fermentation of Lactobacillus acidophilus, but was not applicable to the other strains. Despite being derived from the same source, the FOS from different manufactures showed different effects on the fermentation characteristics of strains, presumably because of the different contents of impurities they had. It was postulated that some prebiotics are beneficial to human health not by directly working on promoting the growth of lactobacilli or bifidobacteria in vivo; instead, they are degraded by other bacteria in the gut before being utilized by probiotic strains.