硬粒小麦缺失4A蜡质蛋白对直链淀粉含量及淀粉品质特性的影响

为了明确硬粒小麦缺失不同Wx蛋白对直链淀粉含量的效应和Wx-B1 缺失型(缺失4A蜡质蛋白)的淀粉特性,利用SDS-PAGE电泳分析技术对1个硬粒小麦F2分离群体(来源于以硬粒小麦品系B413Wx-B1缺失型为母本、PI435045Wx-A1缺失型为父本的杂交组合)的102个单籽粒进行了Wx蛋白的筛选,并测定了其中72个单籽粒(其中Wx-B1 缺失型21粒,Wx-A1 缺失型10粒,正常型41粒)的直链淀粉含量;同时测定了11个Wx-B1 缺失型和7个正常型硬粒小麦品系面粉的直链淀粉含量、膨胀势和RVA黏度.结果表明,硬粒小麦2对Wx基因的分离符合9∶3∶3∶1的分离模式,Wx-B1 缺失型籽粒的直链淀粉含量比正常型低4.78%.Wx-B1 缺失型的面粉直链淀粉含量比正常型低5.76%,RVA高峰黏度和膨胀势比正常型分别高116.02 RVU和3.69 g/g.相关分析表明,直链淀粉含量与RVA高峰黏度和膨胀势均呈极显著负相关.

Effect of Wx-B1 null on Amylose Content and Starch Properties in Durum Wheat

To understand the effects of different Wx protein deficiency on amylose content and the starch properties of Wx-B1 null in durum wheat,102 single half seeds were screened for Wx genotype in a F2 segregating population derived from a cross of two durum wheat lines of B413 with Wx-B1 null and PI435045 with Wx-A1 null as female and male parents,respectively,and meanwhile 72 single half seeds with 21 Wx-B1 null,10 Wx-A1 null and 41 normal genotypes from the F2 population were measured for amylose content.Flours...