山桃生根成苗关键技术研究

山桃是甘肃地区普遍应用的桃树优良砧木，具有抗寒、抗旱的特性。然而山桃通过种子繁殖的方式存在一定比例上的抗性分离现象，而且繁殖周期较长。为探索山桃组培快繁技术。对山桃组培苗的生根培养和炼苗移栽进行了试验。结果表明，以改良QL培养基为基本培养基，当IBA浓度为1.0 mg/L时，试管苗生根效果最好，根系粗壮，侧根较多，生根率达94%以上，平均根数6根以上，平均根长6.8 cm以上；添加80 mg/L PG的处理，无论是生根率、平均根数还是平均根长，较不添加80 mg/L PG的处理相比均表现不理想。将根长超过3 cm的山桃组培苗置于温室中，先遮光炼苗5～7 d，后将瓶盖打开1/2，并配制800倍多菌灵溶液少量倒入组培瓶中，4 d后可将瓶盖全部打开继续炼苗5～7 d，组培苗根系生长基本正常，炼苗成活率可达88%。

Study on the Key Techniques for Rooting and Seedling Formation of Prunus davidiana(Carr.) Franch

Mountain peach, i.e. Prunus davidiana(Carr.) Franch, is a high-quality rootstock which is widely used in Gansu Province, it possesses both cold and drought resistance. However, mountain peach has a percentage of resistance separation in the way of seed propagation, and its propagation cycle is long. To explore the technology of tissue culture and rapid propagation of mountain peach. Rooting culture of the test-tube seedling and the transplanting of the tissue culture seedling of mountain peach were conducted to study the tissue culture and rapid propagation system of mountain peach. The results were as followed. When improved QL culture medium was used, best tube seedlings rooting effect was detected under the concentration of IBA at 1.0 mg/L, which delivered stronger root systemand more capillary roots, the rooting rate was above 94%, the average root number was over 6 and average root length was more than 6.8 cm under this concentration, whereas in the treatment of adding 80 mg/L PG, the average rooting rate, root number and root length showed less ideal performance compared with those in treatment with no 80 mg/L PG added. Tissue culture seedlings with root length over 3 cm were placed in the greenhouse environment, dark cultured for 5 to 7 days first, then small amount of 800 times Carbendazim solution were poured into the tissue culture bottle with lid half open, after 4 days, culture seedling root were refined for 5 to 7 days with cap fully open, in this way, the root system growth of tissue cultured seedlings was basically normal and the survival rate of refined seedlings could reach 88%.