| 壳聚糖固定化木聚糖酶的工艺研究 |
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| 引用本文： | 曹香林,王雅静,仝艳艳,邢 梦,潘 瑞,孙冰潇,聂国兴.壳聚糖固定化木聚糖酶的工艺研究[J].西北农业学报,2011,20(6):190-193. |
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| 作者姓名： | 曹香林 王雅静 仝艳艳 邢 梦 潘 瑞 孙冰潇 聂国兴 |
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| 作者单位： | 河南师范大学生命科学学院,河南新乡,453007 |
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| 基金项目： | 河南省教育厅自然科学研究计划项目，河南省水产养殖学重点学科资助项目，河南师范大学生命科学实验教学示范中心大学生科研创新项目资助 |
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| 摘 要： | 先对影响固定化木聚糖酶效果的几个单因素如壳聚糖质量浓度、戊二醛体积分数、给酶量等指标进行测定,然后采用L9(43)正交试验设计法对壳聚糖作为载体固定化木聚糖酶的工艺进行优化.单因素试验结果表明,当壳聚糖质量浓度为50和60 g/L时,其固定化酶活力较高;而交联剂戊二醛为60 mL/L时,所形成的载体与酶结含量达最大;将...
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| 关 键 词： | 壳聚糖 固定化 木聚糖酶 正交设计 |
Study on the Immobilization Xylanase from Aspergillus niger on Chitosan |
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| CAO Xianglin,WANG Yajing,TONG Yanyan,XING Meng,PAN Rui,SUN Bingxiao and NIE Guoxing.Study on the Immobilization Xylanase from Aspergillus niger on Chitosan[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(6):190-193. |
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| Authors: | CAO Xianglin WANG Yajing TONG Yanyan XING Meng PAN Rui SUN Bingxiao and NIE Guoxing |
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| Institution: | CAO Xianglin,WANG Yajing,TONG Yanyan,XING Meng,PAN Rui,SUN Bingxiao and NIE Guoxing(College of Life Sciences,Henan Normal University,Xinxiang Henan 453007,China) |
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| Abstract: | To optimize the preparation of chitosan microsphere with single factor bolting and orthogona test design,relative activity of immobilized enzymes were studied,xylanase is immobilized on chitosan microsphere by cross linking and adsorbing and the conditions were gained by SPSS software dealing with the date.The single factor experiments showed that activity of immobilized xylanase is better under the condition of 50 g/L and 60 g/L chitosan;the binding capacity between vector and enzyme is obtained at 60 mL/L... |
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| Keywords: | Chitosan Immobilized enzyme Xylanase Orthogona test |
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