| 新梨七号茎段组织培养再生植株的研究 |
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| 引用本文： | 李嫦艳,吴翠云.新梨七号茎段组织培养再生植株的研究[J].西北农业学报,2011,20(5):130-134. |
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| 作者姓名： | 李嫦艳 吴翠云 |
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| 作者单位： | 1. 塔里木大学植物科学学院,新疆阿拉尔,843300
2. 塔里木大学植物科学学院,新疆阿拉尔,843300;新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔,843300 |
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| 摘 要： | 从植物生长调节物质的不同配比、不同部位诱导不定芽以及炼苗移栽等几个方面对新梨七号离体茎段再生进行研究,获得茎段产生愈伤组织及不定芽再生的适宜培养基,分析以上因素对新梨七号再生体系建立的影响。结果表明,新梨七号的茎段最适宜愈伤诱导培养基为MS+2,4-D 1.0mg/L+6-BA 0.5mg/L,愈伤组织诱导率为100%;茎段诱导不定芽的最佳培养基为MS+6-BA 1.0mg/L+NAA 0.5mg/L,诱导率为95%,增殖倍数为7.29;最适宜不定芽生根培养基为1/2MS+IBA 2.5mg/L+蔗糖15g/L,生根率为90%;封口炼苗时间14d为最好,移栽时间7月最佳。
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| 关 键 词： | 新梨七号 茎段 不定芽 暗培养 移栽 |
Regeneration of the Pear Cultivar Xinli No.7 from Stems Culture |
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| LI Changyan and WU Cuiyun.Regeneration of the Pear Cultivar Xinli No.7 from Stems Culture[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(5):130-134. |
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| Authors: | LI Changyan and WU Cuiyun |
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| Institution: | LI Changyan1 and WU Cuiyun1,2 (1.College of Plant Science and Technology College,Alar Xinjiang 843300,China,2.Key Laboratory of Protection and Utilzation of Biological Resources in Tarim Basin,Xinjiang Production&Construction Group,Tarim University,China) |
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| Abstract: | The factors affecting on plant regeneration from stem of pear variety Xinli No.7 in vitro were studied.These factors included ways of stem inoculation,types and concentration of plant growth regulators,explant types,and rooting and transplantation and so on.The result shows that the optimal medium for callus induction was MS + 2,4-D 1.0 mg/L +6-BA 0.5 mg/L,with the callus induction rate of 100%;The optimal medium for plant regeneration was MS supplemented with 6-BA 1.0 mg/L and NAA 0.5 mg/L,regeneration rat... |
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| Keywords: | Xinli No 7 Stem culture Adventitious buds Dark culture Transplant |
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