| 进口猪产品中莱克多巴胺残留的快速检测 |
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| 引用本文： | 陈茹,洪振涛,吴晓薇,王志元,曾碧健,刘志玲,林志雄.进口猪产品中莱克多巴胺残留的快速检测[J].中国兽药杂志,2011,45(8). |
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| 作者姓名： | 陈茹 洪振涛 吴晓薇 王志元 曾碧健 刘志玲 林志雄 |
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| 作者单位： | 1. 广东出入境检验检疫局技术中心,广州,510623
2. 广州市农产品质量安全监督检测中心,广州,510315 |
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| 基金项目： | 致谢:感谢广东出入境检验检疫局技术中心林峰研究员和广州恩康科技有限公司提供技术支持 |
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| 摘 要： | 应用经验证可靠的ELISA方法，对进口猪产品进行莱克多巴胺残留快速筛选检测，对ELISA检测阳性的样品采用色谱质谱联用分析方法进行确证检测。1330份猪产品的检测结果显示，采用ELISA方法检出阳性率达11．4％（152／1330）；对ELISA阳性样品采用高效液相色谱-串联质谱（HPLC—MS／MS）或气相色谱-质谱方法（GC—MS）确证，阳性率达90．8％（138／152）。检测工作表明，综合运用快速筛选检验技术与确证检测技术可实现快速准确检测动物产品中莱克多巴胺残留，提高进出境检验工作效率。
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| 关 键 词： | 莱克多巴胺 猪产品 ELISA 高效液相色谱-串联质谱 气相色谱-质谱 |
Rapid Detection of Ractopamine Residues in Imported Pocine Products |
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| CHEN Ru,HONG Zhen-tao,WU Xiao-wei,WANG Zhi-yuan,ZENG Bi-jian,LIU Zhi-ling,LIN Zhi-xiong.Rapid Detection of Ractopamine Residues in Imported Pocine Products[J].Chinese Journal of Veterinary Drug,2011,45(8). |
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| Authors: | CHEN Ru HONG Zhen-tao WU Xiao-wei WANG Zhi-yuan ZENG Bi-jian LIU Zhi-ling LIN Zhi-xiong |
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| Institution: | CHEN Ru1,HONG Zhen-tao2,WU Xiao-wei1,WANG Zhi-yuan1,ZENG Bi-jian1,LIU Zhi-ling1,LIN Zhi-xiong1(1.Technical Centre of Guangdong Entry-exit Inspection & Quarantine Bureau,Guangzhou 510623,China,2.Guangzhou Agricultural Products Quality and Safety Supervisory Center,Guangzhou 510315,China) |
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| Abstract: | A validated ELISA method was applied to screening detection of ractopamine residues in imported porcine products, and the ELISA positive samples were further determined by chromatography and mass spectromety confirmatory method. The ELISA detected 11.4% （152/1 330） positive from a total of 1 330 porcine raw products, and 90.8% （138/152） samples were confirmed as positive by HPLC -MS/MS or GC -MS methods. The combined application of rapid screening method and confirmation method could achieve rapid and accurate detection of ractopamine residues in animal products and help improve the import and export inspection practices. |
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| Keywords: | ractopamine porcine products ELISA HPLC- MS/MS GC- MS/MS |
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