| 谷物中掺杂油菜籽的转基因成分检测 |
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| 引用本文： | 张舒亚,潘良文.谷物中掺杂油菜籽的转基因成分检测[J].种子,2006,25(4):18-20. |
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| 作者姓名： | 张舒亚 潘良文 |
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| 作者单位： | 上海出入境检验检疫局,上海,200135 |
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| 基金项目： | 国家标准专项上海地方实施项目;上海市科委资助项目 |
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| 摘 要： | 杂质是谷物及其加工产品中转基因成分污染的一个重要来源,本文对来源于进境谷物中的50份油菜籽样品进行了转基因成分（gox基因、pat基因和bar基因）检测.通过常规定性PCR检测,在31份样品中检出转基因油菜籽,检出率为62%.本研究使用ABI 7700定量PCR仪对油菜籽样品DNA进行了实时荧光PCR定性检测分析,在32份样品中检出含有转基因油菜籽,检出率为64%.在今后要加强进出口谷物中杂质作物的转基因成分检测.
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| 关 键 词： | 进境谷物 杂质 转基因油菜籽 PCR检测 |
| 收稿时间： | 2005-12-25 |
| 修稿时间： | 2005-12-25 |
Transgenic Ingredients Detection of GM Rapeseed from Import Grains |
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| Zhang Shuya,Pan Liangwen.Transgenic Ingredients Detection of GM Rapeseed from Import Grains[J].Seed,2006,25(4):18-20. |
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| Authors: | Zhang Shuya Pan Liangwen |
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| Institution: | Shanghai Entry-Exit Inspection and Quarantine Bureau ,Shanghai ,200135 |
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| Abstract: | The presence of transgenes in 50 rapeseed samples from imported grains were determined by conventional PCR and real-time PCR. The conventional qualitative PCR results showed that GMO derived material existed in 31 samples , and the percentage is 62%. Furthermore, real-time qualitative PCR was conducted by ABI 7700 Sequence Detection System, and GMO derived material was found in 32 samples , and the percent- age is 64%. it is necessary to detect transgenes in foreign materials in the future. |
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| Keywords: | Imported grains Foreign materials GM rapeseed PCR detection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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