THRSP基因C184T突变与秦川牛肉质性状相关性的研究

 【目的】研究秦川牛THRSP基因第一外显子184 bp处C/T错义突变与部分肉质性状的关系。【方法】选取405头相同饲养条件下无亲缘关系的18～20月龄的秦川牛,利用PCR-SSCP技术对THRSP基因进行个体基因型分析;运用SPSS统计软件中的GLM模型分析该SNP与93头秦川牛部分肉用性能指标的相关性。【结果】该位点引发THRSP基因第51个氨基酸由缬氨酸（GTG）变为丙氨酸（GCG）。多态信息含量为0.3583,属于中度多态,杂合度为0.4676,有效等位基因数为1.8783。卡方检验显示该位点处于Hardy-Weinberg不平衡状态（P＜0.01）。相关分析结果显示,该位点基因型与嫩度和系水力2个性状显著相关。多重比较结果显示,AA基因型个体的嫩度显著高于AB型（P＜0.05）,BB基因型个体的系水力显著高于AB型（P＜0.05)。【结论】这一位点可能是影响牛肉嫩度及系水力的主效QTN或与之紧密连锁,可做为肉牛肉质选育的候选分子标记。

Correlation of C184T Mutation in THRSP Gene with Meat Traits in the Qinchuan Cattle

[Objective] The study aimed at analysis ofa C/T missense mutation founded by direct sequencing at THRSP geneexonl 184 bp locus and investigations of the correlation of the SNP with some meat traits in Qinchuan cattle. [Method] Four hundred and five Qinchuan cattles without genetic relationship at age of 18-20 months were selected in this study, and the cattle were kept under the same condition. Individual genotypes of THRSP gene were analyzed by PCR-SSCP method.General linear model (GLM) of SPSS statistical software was used to study the correlation between this SNP and partial meat traits of 93 Qinchuan cattle. [Result] This mutation leads to a missense mutation from Val (GTG) to Ala (GCG) at the 51th amino acid. In this locus, PIC (polymorphism information content) is 0.3583, belongs to moderate polymorphisms, He (heterozygosity) is 0.4676, Ne (number of effective allele) is 1.8783. The chi-square test showed that this locus is not in Hardy-Weinberg equilibrium (P<0.01). Correlation analysis revealed that genotype in this locus has a significant correlation with tenderness and WHC (water holding capacity) traits. Multi-comparison indicated that the tenderness of AA genotype individual is significantly higher than that of AB (P<0.05), and the WHC of BB is significantly higher than AB (P<0.05). [Conclusion] This locus is a potential major QTN that affects tenderness and WHC or tightly linked to it, it could be a candidate molecular marker for the beef cattle breeding.