Inverse correlation between mRNA levels in GFP-silenced transgenic Nicotiana benthamiana and resistance to Potato virus X engineered to contain GFP sequence |
| |
Authors: | Masaki Shimono Makoto Ino Shoji Sonoda Tatsuhito Fujimura Masamichi Nishiguchi |
| |
Institution: | (1) National Institute of Agrobiological Sciences, Tsukuba, Japan;(2) Institute of Agricultural and Forest Engineering, University of Tsukuba, Tsukuba, Japan;(3) Present address: Research Institute for Bioresources, Okayama University, Okayama, Japan;(4) Present address: Faculty of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan |
| |
Abstract: | Nicotiana benthamiana was transformed with a green fluorescent protein (GFP) gene driven by cauliflower mosaic virus 35S promoter. A GFP-silenced line and a nonsilenced line were selected after ultraviolet irradiation. GFP short-interfering RNAs (siRNAs) were detected in the silenced line but not in the nonsilenced line. T1 progeny of the silenced line varied in GFP suppression patterns and were grouped into three types (I, II, III) based on the GFP suppression pattern. With Northern blot analysis, different levels of GFP mRNA accumulated, from a very low level in type I and II to an intermediate level in type III, in contrast to a much higher level in the nonsilenced line. Plants were also inoculated with Potato virus X engineered to contain the GFP sequence to evaluate the levels of virus resistance. None to a few GFP spots were observed on inoculated leaves in types I and II, whereas numerous spots and systemic infection appeared in type III. These results showed that virus resistance was inversely correlated with the levels of mRNA, suggesting that the strength of RNA silencing determines the extent of virus resistance. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|