芸薹种作物抽薹相关基因BrFLC1的CAPS标记

以16份芸薹种作物DH系为试材，通过人工春化处理对其抽薹性状进行了鉴定，明确了材料间抽薹早晚存在显著差异；根据BrFLC1基因序列设计引物进行PCR扩增发现，所有16份材料均获得980 bp的片段。多序列比对与抽薹性的关联分析发现，材料的抽薹早晚与扩增片段的碱基变异相关联,并发现关联位点的碱基变异为限制性内切酶Mva I的识别位点；利用该酶对PCR产物酶切可以将材料明显区分开来，从而建立了BrFLC1基因的CAPS标记（命名为G-Mva I）。利用此标记对96份芸薹种作物DH材料的验证表明,该基因的分子标记结果与抽薹时间表型显著相关，用该标记可以进行分子标记辅助选择。

A CAPS Marker Linked to Bolting Related Gene BrFLC1 in Brassica rapa

Bolting time of 16 Brassica rapa accessions were investigated under artificial vernalization treatment. The result showed that there was a wide range variation of bolting time among these accessions. Polymerase Chain reaction (PCR) with a primer pair based on the BrFLC1 gene sequence generated a 980 bp fragment from all accessions. A base variation located at P3 was significantly associated with bolting time, and the base variation at this position is the recognition site of restriction enzyme Mva I. Therefore, a Cleaved Amplified Polymorphic Sequnence (CAPS) marker designated as G-Mva I was developed for this locus by digesting the amplified fragment with Mva I. The marker was verified in 96 B. rapa DH lines, displaying a significant association between the bolting phenotypes and the genotypes of G- Mva I. These results indicated that G-Mva I can be easily used for marker-assisted selection (MAS) in late bolting or bolting-resistance breeding.