|
|||||
|
|
| BrVIN3.1 互作蛋白BrZAT12 在大白菜春化途径中行使功能初探 | |
| 引用本文： | 辛晓云,王斌,赵岫云,张德双,汪维红,余阳俊,苏同兵,于拴仓,张凤兰.BrVIN3.1 互作蛋白BrZAT12 在大白菜春化途径中行使功能初探[J].中国蔬菜,2019,1(11):30-35. |
| 作者姓名： | 辛晓云 王斌 赵岫云 张德双 汪维红 余阳俊 苏同兵 于拴仓 张凤兰 |
| 作者单位： | 1. 北京市农林科学院蔬菜研究中心，北京 100097；;2. 北京市农学院植物与科学技术学院，北京 102206 |
| 基金项目： | 国家自然科学基金项目（31772307），北京市自然科学基金 项目（6172008），北京市农林科学院基金项目（KJCX20180204）， “十三五”国家重点研发计划项目（2016YFD0100506），中国博士后 科学基金项目（2018M641259） |
| 摘 要： | BrVIN3.1 在春化过程中通过调控BrFLC1 的表观修饰状态，进而调控其表达控制大白菜花期。本试验以BrVIN3.1
（Bra020445）为诱饵，进行酵母cDNA 文库筛选，从中筛选到它的互作蛋白BrZAT12（Bra006691）；进一步进行酵母双
杂一对一验证，证明BrZAT12 能够与BrVIN3.1 互作。采用低温处理不同花期的大白菜材料，进行表达模式分析，发现
BrZAT12 的表达模式在抽薹性状不同的大白菜材料中存在差异，在易抽薹材料中的表达量明显高于耐抽薹材料，且在耐抽薹
材料中变化幅度较小，同时BrZAT12 的表达模式与BrVIN3.1 相关。推测BrZAT12 可能在大白菜春化调控的开花途径中行使
功能。 |
| 关 键 词： | 大白菜 BrVIN3.1 BrZAT12 酵母双杂 荧光定量PCR |
Preliminary Studies on The Function of BrZAT12，a Protein Interacted with BrVIN3.1，during Flowering Time in Chinese Cabbage |
|
| XIN Xiao-yun,WANG Bin,ZHAO Xiu-yun,ZHANG De-shuang,WANG Wei-hong,YU Yang-jun,SU Tong-bing,YU Shuan-cang,ZHANG Feng-lan.Preliminary Studies on The Function of BrZAT12，a Protein Interacted with BrVIN3.1，during Flowering Time in Chinese Cabbage[J].China Vegetables,2019,1(11):30-35. | |
| Authors: | XIN Xiao-yun WANG Bin ZHAO Xiu-yun ZHANG De-shuang WANG Wei-hong YU Yang-jun SU Tong-bing YU Shuan-cang ZHANG Feng-lan |
| Institution: | 1. Beijing Vegetable Research Center，Beijing Academy of Agriculture and Forestry Sciences，Beijing 100097，China；;2.College; of Plant Science and Technology，Beijing College of Agriculture，Beijing 102206，China |
| Abstract: | During flowering time process，BrVIN3.1 regulates by mediating the epigenetic states of BrFLC1，regulated its expression then controlled the flowering time of Chinese cabbage．Taking BrVIN3.1 （Bra020445）as bait，the paper carried out screen selection on yeast cDNA library and obtained BrVIN3.1’s interaction protein and BrZAT12（Bra006691）．Yeast-two-hybrid verification was conducted．The results showed that they did interact in vivo．Chinese cabbage materials at different flowering times were treated by refrigeration．Expression pattern was analyzed．The results showed that BrZAT12 expression patterns were different in Chinese cabbage materials at different bolting times．The expression quantities of easy bolting materials were higher than those of tolerant bolting materials，which had lesser amplitude of variation．In summary，the paper speculated that BrZAT12 may function in flowering pathway by regulating the expression of BrVIN3.1 in Chinese cabbage． |
| Keywords: | Chinese cabbage BrVIN3 1 BrZAT12 Yeast double heterozygous Real-time PCR |
| 本文献已被 CNKI 等数据库收录！ | |
| 点击此处可从《中国蔬菜》浏览原始摘要信息 | |
| 点击此处可从《中国蔬菜》下载免费的PDF全文 | |