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| 基因型和采样时期对甜樱桃体细胞胚胎发生的影响 | |
| 引用本文： | 吴雅琴,程和禾,刘国俭,李玉生,吴永杰,赵艳华. 基因型和采样时期对甜樱桃体细胞胚胎发生的影响[J]. 河北农业科学, 2008, 12(11): 33-35 |
| 作者姓名： | 吴雅琴 程和禾 刘国俭 李玉生 吴永杰 赵艳华 |
| 作者单位： | 河北省农林科学院昌黎果树研究所,河北,昌黎,066600;河北省农林科学院昌黎果树研究所,河北,昌黎,066600;河北省农林科学院昌黎果树研究所,河北,昌黎,066600;河北省农林科学院昌黎果树研究所,河北,昌黎,066600;河北省农林科学院昌黎果树研究所,河北,昌黎,066600;河北省农林科学院昌黎果树研究所,河北,昌黎,066600 |
| 基金项目： | 河北省农林科学院资助项目 |
| 摘 要： | 以不同基因型甜樱桃的未成熟胚为材料,从幼胚的采样时期、蔗糖浓度和2,4-D等方面探讨了甜樱桃体细胞胚胎发生的影响因素。结果表明：采样时期在花后35 d左右,幼胚≤5 mm时,胚性愈伤组织形成的频率较高（92.9%）;体细胞胚胎发生的适宜培养基为MS＋2,4-D 2.0 mg/L＋蔗糖40 g/L。 |
| 关 键 词： | 甜樱桃 幼胚 体细胞胚发生 |
Effect of Genotypes and Sampling Dates on Somatic Embryogenesis of Sweet Cherry |
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| WU Ya-qin,CHENG He-he,LIU Guo-jian,LI Yu-sheng,WU Yong-jie,ZHAO Yan-hua. Effect of Genotypes and Sampling Dates on Somatic Embryogenesis of Sweet Cherry[J]. Journal of Hebei Agricultural Sciences, 2008, 12(11): 33-35 | |
| Authors: | WU Ya-qin CHENG He-he LIU Guo-jian LI Yu-sheng WU Yong-jie ZHAO Yan-hua |
| Affiliation: | WU Ya-qin,CHENG He-he,LIU Guo-jian,LI Yu-sheng,WU Yong-jie,ZHAO Yan-hua* |
| Abstract: | The immature of different genotypes of sweet cherry were used as materials.The influencing factors to the embryogenesis of sweet cherry were analyzed including sampling dates of young embryo,concentrations of sucrose and 24-D.When the sampling dates was about 35 d after flower and the size of young embryo was less than 5 mm,the high frequency 92.9% of the embryogenesis callus was observed.The optimal culture medium was MS 24-D 2.0 mg/L sucrose 40 mg/L. |
| Keywords: | Sweet cherry Young embryo Embryogenesis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |