| 植物内生细菌在棉花体内的定殖动态及对棉花黄萎病的生物防治效果 |
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| 引用本文： | 王娜,陶伟,陈双林,闫淑珍.植物内生细菌在棉花体内的定殖动态及对棉花黄萎病的生物防治效果[J].植物保护学报,2016,43(2):207-214. |
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| 作者姓名： | 王娜 陶伟 陈双林 闫淑珍 |
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| 作者单位： | 南京师范大学生命科学学院,江苏省微生物与功能基因组学重点实验室,江苏省微生物资源产业化工程技术研究中心,南京210046 |
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| 基金项目： | 江苏省自然科学基金(BK2012848) |
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| 摘 要： | 为明确植物内生细菌在棉花体内的定殖规律及对棉花黄萎病防治效果的影响,采用对峙培养法筛选对大丽轮枝菌Verticillium dahliae Kleb.抑菌活性强的植物内生细菌,通过形态及生理生化特征并结合16S r DNA和gyr B基因分析鉴定菌株;利用抗生素标记法及盆栽试验研究其在棉花体内的定殖动态及对棉花黄萎病的防治效果。结果表明:共筛选到3株对大丽轮枝菌具有强抑菌活性的菌株SZ5、DP10和CCM9,其中SZ5为蜡样芽胞杆菌Bacillus cereus,定殖在棉花根部、茎部和叶部,DP10和CCM9为枯草芽胞杆菌B.subtilis,定殖在棉花根部和茎部,3株菌在棉花根部的定殖数量均在10~3CFU/g以上;棉花苗沾根分别接入3株内生细菌10 d后再接种大丽轮枝菌孢子悬浮液,对棉花黄萎病的防治效果达79.52%~89.79%,而3株内生细菌沾根接种后立即移栽到含有大丽轮枝菌的土壤中对棉花黄萎病的防治效果为23.77%~36.03%。表明不同内生细菌菌株在棉花体内的定殖规律不同;且在棉苗中定殖一定数量后,才能对棉花黄萎病产生较高的防治效果。
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| 关 键 词： | 内生细菌 棉花黄萎病 定殖动态 生物防治 |
| 收稿时间： | 2014/10/28 0:00:00 |
Colonization trends of endophytic bacteria in cotton and their biological control effect on cotton Verticillium wilt |
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| Wang N,Tao Wei,Chen Shuanglin and Yan Shuzhen.Colonization trends of endophytic bacteria in cotton and their biological control effect on cotton Verticillium wilt[J].Acta Phytophylacica Sinica,2016,43(2):207-214. |
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| Authors: | Wang N Tao Wei Chen Shuanglin and Yan Shuzhen |
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| Institution: | Jiangsu Key Laboratory for Microbes and Functional Genomics; Jiangsu Provincial Engineering and Technology Research Center for Microbiology Research; College of Life Sciences, Nanjing Normal University, Nanjing 210046, Jiangsu Province, China,Jiangsu Key Laboratory for Microbes and Functional Genomics; Jiangsu Provincial Engineering and Technology Research Center for Microbiology Research; College of Life Sciences, Nanjing Normal University, Nanjing 210046, Jiangsu Province, China,Jiangsu Key Laboratory for Microbes and Functional Genomics; Jiangsu Provincial Engineering and Technology Research Center for Microbiology Research; College of Life Sciences, Nanjing Normal University, Nanjing 210046, Jiangsu Province, China and Jiangsu Key Laboratory for Microbes and Functional Genomics; Jiangsu Provincial Engineering and Technology Research Center for Microbiology Research; College of Life Sciences, Nanjing Normal University, Nanjing 210046, Jiangsu Province, China |
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| Abstract: | |
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| Keywords: | endophytic bacterium cotton Verticillium wilt colonization dynamics biological control |
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