不同锌源对断奶小鼠生长及机体抗氧化能力的影响

选用断奶小鼠72只作为研究对象,随机分为3组(对照组,硫酸锌组和蛋氨酸锌组),每组设4个重复。测定体重、组织锌含量及相关生理生化指标,以研究硫酸锌、蛋氨酸锌2种不同锌供给形式对机体的生长效应及抗氧化能力的影响。结果表明,硫酸锌组和蛋氨酸锌组均能不同程度地提高小鼠体重,蛋氨酸锌组小鼠体重显著高于对照组与硫酸锌组(P<0.05)。饲粮中添加硫酸锌可提高小鼠肝脏和血清中的锌含量(P>0.05);蛋氨酸锌组小鼠肝脏和血清锌含量高于对照组(P<0.05)及硫酸锌组(P>0.05)。2种锌源均显著提高碱性磷酸酶(AKP)、谷胱甘肽过氧化物酶(GSHPx)活性(P<0.05),不同锌源间无明显差异(P>0.05)。添加不同的锌源不同程度地提高总抗氧化能力(TAOC)、总SOD(TSOD)、铜锌超氧化物歧化酶(CuZnSOD)活性(P<0.05),蛋氨酸锌组TAOC、TSOD显著高于硫酸锌组(P<0.05)。添加锌显著降低NO含量(P<0.05),蛋氨酸锌效果显著强于硫酸锌(P<0.05);一氧化氮合酶(NOS)活性表现出与NO相反的趋势(P>0.05)。

Effect of different zinc sources on growth and the ability of antioxidation of mice

This experiment was conducted to investigate the effect of zinc sulfate and zinc methionine on growth and redox of weanling mice (KM). Seventy-two weanling mice were allotted at random into 3 groups with 4 replicates, 6 mice in each replicate. The mice of group 1 were fed with Corn Starch basal diet and group 2 and 3 were supplemented with zinc in 30mg/kg in zinc sulfate and zinc-methionine. Weight gain, activity of alkaline phosphatase, nitric oxide synthase (NOS), glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD) and CuZn-SOD, the content of nitric oxide (NO), serum and liver zinc were determined. The result indicated that both zinc sulfate and zinc methionine improved growth of mice (P>0.05 for ZnSO_4, P<0.05 for Zn-Met), Zn-Met improved growth more effectively than ZnSO_4. Both ZnSO_4 and Zn-Met enhanced serum and liver zinc content (P>0.05 for ZnSO_4, P<0.05 for Zn-Met). The activities of AKP、 GSH-Px were enhanced significantly (P<0.05) showing no difference in two zinc sources. T-AOC, T-SOD and CuZn-SOD were increased by zinc supplementation (P<0.05), and zinc methionine exerted effectively than zinc sulfate (P<0.05). The content of NO was decreased with zinc supplementation (P<0.05), and Zn-Met was more effective than ZnSO_4 (P<0.05). The activity of NOS was increased slightly by zinc supplementation (P>0.05).