| 感染蜜蜂及熊蜂的微孢子虫检测与鉴定方法 |
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| 引用本文： | 陈文锋,吴杰,李继莲.感染蜜蜂及熊蜂的微孢子虫检测与鉴定方法[J].中国农学通报,2010,26(19):8-12. |
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| 作者姓名： | 陈文锋 吴杰 李继莲 |
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| 作者单位： | 中国农业科学院蜜蜂研究所,农业部授粉昆虫生物学重点开放实验室,北京,100093 |
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| 基金项目： | 国家蜜蜂产业技术体系建设专项经费项目，国家自然科学基金项目"不同寄主熊蜂微孢子虫的亲缘关系"，中瑞政府间科技合作项目"中国和欧洲熊蜂的微孢子虫的生物多样性、危害及防治" |
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| 摘 要： | 微孢子虫是一类专性细胞内寄生的单细胞生物，是蜜蜂和熊蜂的主要寄生虫之一，对蜜蜂和熊蜂养殖危害较大。本文总结了感染蜜蜂及熊蜂的微孢子虫检测与鉴定方法，主要包括常规生物学方法、以PCR技术为基础的分子生物学方法和免疫学方法等。这些方法对蜜蜂及熊蜂微孢子虫的研究及防治发挥着重要的作用。
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| 关 键 词： | 木霉 木霉 紫外诱变 抗生素 筛选 |
| 收稿时间： | 4/8/2010 12:00:00 AM |
| 修稿时间： | 2010/5/31 0:00:00 |
Detection and Identification methods of Microsporidia in Honeybees and Bumblebees |
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| Wu Jie,Chen Wenfeng,Li Jilian.Detection and Identification methods of Microsporidia in Honeybees and Bumblebees[J].Chinese Agricultural Science Bulletin,2010,26(19):8-12. |
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| Authors: | Wu Jie Chen Wenfeng Li Jilian |
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| Institution: | ( Institute of Apicultural , Chinese Academy of Agricultural Sciences , Key laboratory for Insect-Pollinator Biology of the Ministry of Agriculture , Beijing 100093) |
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| Abstract: | The microsporidia are obligate intracellular parasites that infect many animals including honeybees and bumblebees, and harm to the rearing of honeybees and bumblebees. Here, we reviewed the different detection and identification methods of microsporidia in honeybees and bumblebees. These methods contained the conventional biological methods, the molecular biology methods which based on PCR technique, the immunological methods and so on. All of these methods played an important role in the research and prevention of microsporidia in honeybees and bumblebees. |
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| Keywords: | honeybee bumblebee microsporidia |
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