| 高质量枣基因组DNA提取方法 |
| |
| 引用本文： | 王永康,王永勤,田建保,樊新平,李登科,隋串玲,黄丛林.高质量枣基因组DNA提取方法[J].果树学报,2006,23(2):310-312. |
| |
| 作者姓名： | 王永康 王永勤 田建保 樊新平 李登科 隋串玲 黄丛林 |
| |
| 作者单位： | 1. 山西省农业科学院果树研究所,山西太谷,030815
2. 北京市农林科学院北京农业生物技术研究中心,北京,100089 |
| |
| 基金项目： | 科技部科技基础条件平台建设计划
,
农业部作物种质资源保护项目 |
| |
| 摘 要： | 主要针对枣组织中多糖严重干扰基因组DNA提取质量的问题,比较了常规CTAB法、TE3D法和改良CTAB法3种方法的处理效果。结果表明:常规CTAB法提取DNA难以除去多糖类杂质;TE3D法提取的DNA多糖杂质少,但产率低,易降解,褐化严重;改良CTAB法提取DNA产率高,无明显降解,杂质少,D260nm/D280nm比值1.80左右,通过基因组DNA-AFLP指纹图谱分析,完全满足实验要求。并对改良CTAB法的关键步骤作了具体分析讨论。
|
| 关 键 词： | 枣树 DNA提取 改良CTAB法 |
| 文章编号： | 1009-9980（2006）01-310-03 |
| 收稿时间： | 2005-06-10 |
| 修稿时间： | 2005-10-17 |
Extraction methods of high quality DNA from Zizyphus jujuba |
| |
| WANG Yong-kang,WANG Yong-qin,TIAN Jian-bao,FAN Xin-ping,LI Deng-ke,SUI Chuan-ling,HUANG Cong-lin.Extraction methods of high quality DNA from Zizyphus jujuba[J].Journal of Fruit Science,2006,23(2):310-312. |
| |
| Authors: | WANG Yong-kang WANG Yong-qin TIAN Jian-bao FAN Xin-ping LI Deng-ke SUI Chuan-ling HUANG Cong-lin |
| |
| Institution: | 1.Rresearch Institute of Pomology, Shanxi Academy of Agricultural Science, Taigu, Shanxi 030815 China; 2.Beijing Agro-Biotechnology Research Center, Beijing 100089 China |
| |
| Abstract: | In order to solve the problem of polyhexose interfering the genomic DNA extraction of Zizyphus jujuba Mill. , 3 methods, the conventional CTAB, TE3D and improved CTAB were compared. The results showed that the conventional CTAB method was difficult to get rid of polyhexose completely; the TE3D method could remove polyhexose effectively, but extraction rate was very low, DNA browned and degraded seriously; the improved CTAB method was an effective way to solve all kinds of problem, DNA concentration was up to 1000 ng/μL, degradation was light and impurity was little, A260/A280 was 1.80 or so. When tested through the DNA-AFLP fingerprinting, they could meet experiment requirement perfectly. In addition, the key steps and main features of the improved CTAB method are discussed. |
| |
| Keywords: | Zizyphus jujuba Mill DNA extraction Improved CTAB method |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
