六个品种猪胰岛素抵抗素基因单核苷酸多态性分析

目的 探讨胰岛素抵抗素基因是否存在单核苷酸多态性，及其与肥胖、脂肪沉积的相关性。方法 本试验选择纯种蓝塘猪、陆川猪、临高猪、长白猪、大白猪、杜洛克，采用PCR-SSCP方法，结合相关测定指标做进一步基因分析。结果 在供试的6种纯种猪中抵抗素基因启动子检测到CC、TT、CT 3种基因型，内含子-1有EE、FF、GG、EF、EG、FG 6种基因型，内含子-2有LL、MM、NN、LM、LN、MN 6种基因型，外显子-2有CC、GG 2种基因型，基因频率与脂肪沉积量分析表明两者存在相关。结论 抵抗素基因存在单核苷酸多态性，且与脂肪沉积调控有关。

SNP Analysis of Resistin Gene in Six Pig Breeds by PCR-SSCP

Objective To explore the correlation between the polymorphism of Single nucleotide polymorphisms(SNPs) of Resistin and the reguration of fat diposition. Methods Screening formutations in the entire coding region for the Resisin gene were Performed by the polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) in Lantang pig、Luchuan pig、Lingao pig、Landrace、Large white and duroc pigs. Results Detected results of genotype demonstrate that no polymorphism in exon-1 and exon-3,two genotypes in exon-2(CC、GG),three genotypes in promoter region(CC、TT、CT),six genotypes in intron-1(EE、FF、GG、EF、EG、FG) and intron-2(LL、MM、NN、LM、LN、MN). Variance analysis revealed that Resistin gene had a high linkage with their growth and fatness traits.Conclusion There are SNPs in resistin gene in pigs ,and have association of these SNPs with fat diposition and obesity in pigs.