| 银龙鱼烂鳃病病原的分离和鉴定 |
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| 引用本文： | 史谢尧,贾文平,郝爽,张振国,罗璋,周文礼,冯守明.银龙鱼烂鳃病病原的分离和鉴定[J].安徽农业科学,2018,46(15):75-78. |
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| 作者姓名： | 史谢尧 贾文平 郝爽 张振国 罗璋 周文礼 冯守明 |
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| 作者单位： | 天津农学院水产学院,天津市水产生态及养殖重点实验室,天津300384;天津市水产研究所,天津300221;天津市水产研究所,天津,300221;天津农学院水产学院,天津市水产生态及养殖重点实验室,天津300384;天津市水生动物疫病预防控制中心,天津,300221 |
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| 基金项目： | 天津市水产产业技术体系创新团队项目(ITTFR 2017008)，天津市自然科学基金项目(16JCYBJC30000)，天津市农业科技成果转化与推广项目(201501030) |
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| 摘 要： | 目的]对患烂鳃病银龙鱼开展病原分离和鉴定。方法]采用人工回归感染试验确定分离菌株的致病性,利用生理生化试验和16S r DNA基因序列分析对病原菌进行鉴定。结果]分离菌株YY-2017-3对斑马鱼具有致病性,其生理生化试验结果与柱状黄杆菌相一致;YY-2017-3菌株的16S r DNA基因序列与柱状黄杆菌聚为一支,对多种药物显示耐药。结论]菌株YY-2017-3被鉴定为柱状黄杆菌,这是柱状黄杆菌引起银龙鱼烂鳃病在国内的首次报道。
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| 关 键 词： | 银龙鱼 烂鳃病 病原菌 柱状黄杆菌 |
Isolation and Identification of Pathogen from Osteoglossum bicirrhosum with Gill Rot Disease |
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| Abstract: | Objective]To make the isolation and identification of pathogen from Osteoglossum bicirrhosum with gill rot disease.Method]The pathogenicity of isolated strain was confirmed by artificial infection test,and the isolated strain was identified through physiological and biochemi-cal tests,16S rDNA gene sequencing results.Result]The isolated strain YY-2017-3 was pathogenic to zebra fish,and its physiological and bio-chemical properties were consistent with those of Flavobacterium columnare.YY-2017-3 strain formed a single cluster with 16S rDNA gene se-quences of F.columnare,and it showed resistant to many kinds of antibiotics.Conclusion]YY-2017-3 strain was identified as F.columnare.It was the first report about this bacterium caused gill roll disease of F.columnare in China. |
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