| 紫丁香苷抗肿瘤活性筛选及作用机制研究 |
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| 引用本文： | 秦湫红,朱爱华.紫丁香苷抗肿瘤活性筛选及作用机制研究[J].安徽农业科学,2018,46(14):107-108,112. |
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| 作者姓名： | 秦湫红 朱爱华 |
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| 作者单位： | 陕西新药技术开发中心,陕西西安710075;陕西省创新药物研究中心,陕西西安710075;陕西省中药与天然药物研发重点实验室,陕西西安710075;陕西新药技术开发中心,陕西西安710075;陕西省创新药物研究中心,陕西西安710075;陕西省中药与天然药物研发重点实验室,陕西西安710075 |
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| 基金项目： | 陕西省青年科技新星计划项目(2016KJXX-31) |
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| 摘 要： | 目的]筛选对紫丁香苷较敏感的人肿瘤细胞系,研究其抗癌机理。方法]采用四氮唑盐法(MTT法)测定药物的体外抑制作用,通过测定Caspase-3活性研究细胞凋亡通路在抗癌活性中的作用。结果]在常见的15种人肿瘤细胞中,紫丁香苷的半数抑制浓度IC50低于100μg/m L的有人肝癌细胞HepG2以及人前列腺癌细胞PC-3,紫丁香苷可促进肿瘤细胞中细胞凋亡因子Caspase-3活性增加。结论]人肝癌细胞HepG2以及人前列腺癌细胞PC-3对紫丁香苷较为敏感,紫丁香苷的抑制作用呈现出剂量依赖的特点;细胞凋亡途径在紫丁香苷的抗肿瘤活性中起着一定的作用。
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| 关 键 词： | 紫丁香苷 抗肿瘤 细胞凋亡 |
Study on the Screening and Mechanism of Syringin Anticancer Activity |
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| QIN Qiu-hong,ZHU Ai-hua.Study on the Screening and Mechanism of Syringin Anticancer Activity[J].Journal of Anhui Agricultural Sciences,2018,46(14):107-108,112. |
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| Authors: | QIN Qiu-hong ZHU Ai-hua |
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| Abstract: | Objective]To screen human tumor cell lines sensitive to syringin, and study its anticancer mechanism. Method]To determine the inhibitory effect of syringin in vitro using tetrazolium salt(MTT) assay,and study the role of apoptosis pathway by assay of Caspase-3 ac-tivity. Result]Among the 15 common human tumor cell lines,syringin IC50of hepatoma cell HepG2 and prostate cancer cell PC-3 were below 100 μg/mL. Syringin can promote the activity of Caspase-3 in tumor cells. Conclusion] Hepatoma cell HepG2 and prostate cancer cell PC-3 are more sensitive to syringin,and the inhibition of syringin presents a dose-dependent manner. The cell apoptosis pathway plays a role in the anticancer activity of syringin. |
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