| Real-time PCR方法检测肉品中的沙门氏菌 |
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| 引用本文： | 方平,杨永莉,杨宝,王晓闻. Real-time PCR方法检测肉品中的沙门氏菌[J]. 山西农业科学, 2010, 38(8): 71-76. DOI: 10.3969/j.issn.1002-2481.2010.08.20 |
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| 作者姓名： | 方平 杨永莉 杨宝 王晓闻 |
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| 作者单位： | 山西农业大学食品科学与工程学院,山西,太谷,030801 |
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| 基金项目： | 山西省科技攻关项目，山西高校科技研究开发项目 |
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| 摘 要： | 应用SYBR Green I染料能选择性结合双链DNA的特点,可检测到沙门氏菌fimI基因特异性靶序列扩增所产生的荧光信号,通过熔解曲线可知其熔点值约为85.6℃,而对其他非沙门氏菌则检测不到荧光信号。建立了一种肉品中的沙门氏菌Real-time PCR检测方法,用该方法检测市售牛肉、香肠中的沙门氏菌,其检测灵敏度分别为13,12 cfu/25 g,从样品的处理到得出检验结果可以在10 h内完成。该检测方法具有简便、快速、特异性强、敏感度高等特点。
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| 关 键 词： | 沙门氏菌 Real-time PCR 快速检测 肉品 |
Real-time PCR Assays for Detection of Salmonella in Meat Products |
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| FANG Ping,YANG Yong-li,YANG Bao,WANG Xiao-wen. Real-time PCR Assays for Detection of Salmonella in Meat Products[J]. Journal of Shanxi Agricultural Sciences, 2010, 38(8): 71-76. DOI: 10.3969/j.issn.1002-2481.2010.08.20 |
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| Authors: | FANG Ping YANG Yong-li YANG Bao WANG Xiao-wen |
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| Affiliation: | (College of Food Science and Engineering,Shanxi Agricultural University,Taigu 030801,China) |
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| Abstract: | As SYBR Green Ⅰ could selectively bind to double-stand DNA,it is possible to identify the fimI gene in Salmonella through fluorescence and to confirm the melting point of about 85.6 ℃ through the melting curve,while other non-Salmonella had no fluorescence.And Real-time PCR assay was developed for the detection of Salmonella in meat products.The detection limits of Real-time PCR assay were 13 cfu/25 g beef and 12 cfu/25 g sausage.The entire detection of Salmonella for samples could be completed within 10 hours or even less from the enrichment to the showing the results.It is concluded that PCR is a specific,sensitive,rapid,and convenient method for detection of Salmonella. |
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| Keywords: | Real-time PCR |
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